ArticleBritish journal of pharmacology2003
Extracellular ATP and adenosine induce cell apoptosis of human hepatoma Li-7A cells via the A3 adenosine receptor.
Article in British journal of pharmacology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Deciphering adenosine signaling in hepatocellular carcinoma: Pathways, prognostic models, and therapeutic implications.Clinical and molecular hepatology · 2025Review
- Article
- The Proteomics and Metabolomics Analysis for Screening the Molecular Targets of Action of β-Eudesmol in Cholangiocarcinoma.Asian Pacific journal of cancer prevention : APJCP · 2021Article
- A Review ofFrontiers in veterinary science · 2021Review
- Host CD39 Deficiency Affects Radiation-Induced Tumor Growth Delay and Aggravates Radiation-Induced Normal Tissue Toxicity.Frontiers in oncology · 2020Article
- Effects Of Adenosine On Apoptosis Of Ovarian Cancer A2780 Cells Via ROS And Caspase Pathways.OncoTargets and therapy · 2019Article
- Article
- Prognostic value of purinergic P2X7 receptor expression in patients with hepatocellular carcinoma after curative resection.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Adenosine uptake is the major effector of extracellular ATP toxicity in human cervical cancer cells.Molecular biology of the cell · 2014Article
- Purinergic signalling in the liver in health and disease.Purinergic signalling · 2014Review
- Immunity, inflammation and cancer: a leading role for adenosine.Nature reviews. Cancer · 2013Review
- Purinergic signalling and cancer.Purinergic signalling · 2013Review
- Coexpression of ecto-5'-nucleotidase/CD73 with specific NTPDases differentially regulates adenosine formation in the rat liver.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Inhibition of ecto-ATPase activity by curcumin in hepatocellular carcinoma HepG2 cells.The journal of physiological sciences : JPS · 2012Article
- NTPDase1 governs P2X7-dependent functions in murine macrophages.European journal of immunology · 2010Article
- OppA, the ecto-ATPase of Mycoplasma hominis induces ATP release and cell death in HeLa cells.BMC microbiology · 2008Article
- The Anti-Cancer Effect of AImmunology, endocrine & metabolic agents in medicinal chemistry · 2007Article
- Adenosine protects against suicidal erythrocyte death.Pflugers Archiv : European journal of physiology · 2007Article
- Some facts and thoughts: p73 as a tumor suppressor gene in the network of tumor suppressors.Molecular cancer · 2007Article
- p53 gene in treatment of hepatic carcinoma: status quo.World journal of gastroenterology · 2007Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Extracellular ATP is a potent signaling molecule that modulates a myriad of cellular functions through the activation of P2 purinergic receptors and is cytotoxic to a variety of cells at higher concentrations. The mechanism of ATP-elicited cytotoxicity is not fully understood. In this study, we investigated the effect of extracellular ATP on the human hepatoma Li-7A cells. 2. We observed a time- and dose-dependent growth inhibition of Li-7A cells by ATP, which is accompanied by an increase in the active form of caspase-3 as well as increased cleavage of its substrate, poly (ADP-ribose) polymerase. The cytotoxic effect of extracellular ATP was not mediated by the P2X7 receptor, since (1).the effect was not abolished by the P2X7 receptor antagonists oxidized ATP and KN-62, and (2).extracellular ADP, AMP, and adenosine were also cytotoxic. 3. We found that ATP and ADP were degraded to adenosine by Li-7A cells and that treatment of Li-7A cells by adenosine resulted in growth inhibition and caspase-3 activation, indicating that adenosine is the apoptotic agent. Using adenosine receptor agonists and antagonists, as well as inhibitors of adenosine transport and deamination, we showed that the cytotoxic effect of adenosine is specifically mediated by the A3 receptor even though transcripts of A1, A2A, A2B, and a splice variant of the P2X7 receptors were detected in Li-7A cells by RT-PCR. 4. Cytotoxicity caused by exogenous ATP and adenosine was completely abolished by the caspase-3 inhibitor Z-DEVD-FMK, demonstrating the central role of caspase-3 in apoptosis of Li-7A cells.
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