ArticleMolecular biology of the cell2003
Direct binding of the ligand PSG17 to CD9 requires a CD9 site essential for sperm-egg fusion.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 68 citations in OpenAlex.
- Smart exosomes enhance PDAC targeted therapy.Journal of controlled release : official journal of the Controlled Release Society · 2024Article
- Where are all the egg genes?Frontiers in cell and developmental biology · 2023Review
- Oocyte ERM and EWI Proteins Are Involved in Mouse Fertilization.Frontiers in cell and developmental biology · 2022Article
- The immune-modulating pregnancy-specific glycoproteins evolve rapidly and their presence correlates with hemochorial placentation in primates.BMC genomics · 2021Article
- CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization.International journal of molecular sciences · 2018Article
- Sperm-egg fusion: a molecular enigma of mammalian reproduction.International journal of molecular sciences · 2014Review
- A transgenic insertion on mouse chromosome 17 inactivates a novel immunoglobulin superfamily gene potentially involved in sperm-egg fusion.Mammalian genome : official journal of the International Mammalian Genome Society · 2014Article
- Article
- Construction and Analysis of the Cell Surface's Protein Network for Human Sperm-Egg Interaction.ISRN bioinformatics · 2013Article
- Impaired cell adhesion, apoptosis, and signaling in WASP gene-disrupted Nalm-6 pre-B cells and recovery of cell adhesion using a transducible form of WASp.International journal of hematology · 2012Article
- Functional interplay between tetraspanins and proteases.Cellular and molecular life sciences : CMLS · 2011Review
- Article
- CD9 tetraspanin generates fusion competent sites on the egg membrane for mammalian fertilization.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.Infection and immunity · 2011Article
- Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm.Molecular reproduction and development · 2009Article
- Murine pregnancy-specific glycoprotein 23 induces the proangiogenic factors transforming-growth factor beta 1 and vascular endothelial growth factor a in cell types involved in vascular remodeling in pregnancy.Biology of reproduction · 2008Article
- Targeting of tetraspanin proteins--potential benefits and strategies.Nature reviews. Drug discovery · 2008Review
- CD9 clustering and formation of microvilli zippers between contacting cells regulates virus-induced cell fusion.Traffic (Copenhagen, Denmark) · 2008Article
- Tetraspanins: Small transmembrane proteins with big impact on membrane microdomain structures.Communicative & integrative biology · 2008Article
- Functional implications of tetraspanin proteins in cancer biology.Cancer science · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The function currently attributed to tetraspanins is to organize molecular complexes in the plasma membrane by using multiple cis-interactions. Additionally, the tetraspanin CD9 may be a receptor that binds the soluble ligand PSG17, a member of the immunoglobulin superfamily (IgSF)/CEA subfamily. However, previous data are also consistent with the PSG17 receptor being a CD9 cis-associated protein. In the current study, CD9 extracellular loop (EC2) specifically bound to PSG17-coated beads, indicating a direct interaction between the two proteins. However, CD9-EC2 did not bind to PSG17-coated beads if the CD9-EC2 had the mutation SFQ (173-175) to AAA, a previously studied mutation in egg CD9 that abolishes sperm-egg fusion. Also, PSG17 bound to 293 T cells transfected with wild-type CD9 but not the mutant CD9. By immunofluorescence, PSG17 bound to wild-type eggs but not to CD9 null eggs. The presence of approximately 2 microM recombinant PSG17 produced a significant and reversible inhibition (60-80%) of sperm-egg fusion. Thus, we conclude that CD9 is a receptor for PSG17 and when the PSG17 binding site is mutated or occupied, sperm-egg fusion is impaired. These findings suggest that egg CD9 may function in gamete fusion by binding to a sperm IgSF/CEA subfamily member and such proteins have previously been identified on sperm.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.