ArticleMolecular biology of the cell2004
The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 66 citations in OpenAlex.
- Acentric chromosome congression and alignment on the metaphase plate via kinetochore-independent forces.Genetics · 2025Article
- Astral microtubule cross-linking safeguards uniform nuclear distribution in the Drosophila syncytium.The Journal of cell biology · 2022Article
- Self-Organization of Minimal Anaphase Spindle Midzone Bundles.Current biology : CB · 2019Article
- Chromokinesins.Current biology : CB · 2018Review
- Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes.Aging and disease · 2018Article
- The chromokinesin Klp3a and microtubules facilitate acentric chromosome segregation.The Journal of cell biology · 2017Article
- Screening and identification of key genes regulating fall dormancy in alfalfa leaves.PloS one · 2017Article
- Anaphase B.Biology · 2016Review
- The microtubule cross-linker Feo controls the midzone stability, motor composition, and elongation of the anaphase B spindle in Drosophila embryos.Molecular biology of the cell · 2015Article
- Elevated polar ejection forces stabilize kinetochore-microtubule attachments.The Journal of cell biology · 2013Article
- Kinesin 5B (KIF5B) is required for progression through female meiosis and proper chromosomal segregation in mitotic cells.PloS one · 2013Article
- Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase IIα.The Journal of cell biology · 2012Article
- Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis.The Journal of cell biology · 2012Article
- The novel functions of kinesin motor proteins in plants.Protoplasma · 2012Review
- Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles.Cell division · 2011Article
- Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation.The Plant cell · 2011Article
- A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophila embryos.Journal of cell science · 2010Article
- Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope.The Journal of cell biology · 2010Article
- Microinjection techniques for studying mitosis in the Drosophila melanogaster syncytial embryo.Journal of visualized experiments : JoVE · 2009Article
- Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis.Molecular biology of the cell · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Mitosis requires the concerted activities of multiple microtubule (MT)-based motor proteins. Here we examined the contribution of the chromokinesin, KLP3A, to mitotic spindle morphogenesis and chromosome movements in Drosophila embryos and cultured S2 cells. By immunofluorescence, KLP3A associates with nonfibrous punctae that concentrate in nuclei and display MT-dependent associations with spindles. These punctae concentrate in indistinct domains associated with chromosomes and central spindles and form distinct bands associated with telophase midbodies. The functional disruption of KLP3A by antibodies or dominant negative proteins in embryos, or by RNA interference (RNAi) in S2 cells, does not block mitosis but produces defects in mitotic spindles. Time-lapse confocal observations of mitosis in living embryos reveal that KLP3A inhibition disrupts the organization of interpolar (ip) MTs and produces short spindles. Kinetic analysis suggests that KLP3A contributes to spindle pole separation during the prometaphase-to-metaphase transition (when it antagonizes Ncd) and anaphase B, to normal rates of chromatid motility during anaphase A, and to the proper spacing of daughter nuclei during telophase. We propose that KLP3A acts on MTs associated with chromosome arms and the central spindle to organize ipMT bundles, to drive spindle pole separation and to facilitate chromatid motility.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.