ArticleJournal of clinical microbiology1992
Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.
Article in Journal of clinical microbiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed.
- CanID-PCR: a quick and low-cost PCR tool to identifyMicrobiology spectrum · 2026Article
- Clonal Dissemination of Antifungal-Resistant Candida haemulonii, China.Emerging infectious diseases · 2023Article
- Genetic Diversity and Antifungal Susceptibility of Candida parapsilosis Sensu Stricto Isolated from Bloodstream Infections in Turkish Patients.Mycopathologia · 2018Article
- Characterization of beta-tubulin DNA sequences withinCurrent medical mycology · 2018Article
- Effective inactivation of Saccharomyces cerevisiae in minimally processed Makgeolli using low-pressure homogenization-based pasteurization.SpringerPlus · 2015Article
- Designing a SCAR molecular marker for monitoring Trichoderma cf. harzianum in experimental communities.Journal of Zhejiang University. Science. B · 2014Article
- Epidemiology and echinocandin susceptibility of Candida parapsilosis sensu lato species isolated from bloodstream infections at a Spanish university hospital.The Journal of antimicrobial chemotherapy · 2012Article
- Accurate identification of Candida parapsilosis (sensu lato) by use of mitochondrial DNA and real-time PCR.Journal of clinical microbiology · 2012Article
- Evaluation of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Article
- Prospective multicenter study of the epidemiology, molecular identification, and antifungal susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis isolated from patients with candidemia.Antimicrobial agents and chemotherapy · 2011Article
- Epidemiology and microbiology of nosocomial pediatric candidemia at a northern Indian tertiary care hospital.Mycopathologia · 2011Article
- Assessment of two new molecular methods for identification of Candida parapsilosis sensu lato species.Journal of clinical microbiology · 2011Article
- Typing Candida albicans oral isolates from healthy brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- New polymorphic microsatellite markers able to distinguish among Candida parapsilosis sensu stricto isolates.Journal of clinical microbiology · 2010Article
- Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation.BMC microbiology · 2009Article
- Prevalence, distribution, and antifungal susceptibility profiles of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis in a tertiary care hospital.Journal of clinical microbiology · 2009Article
- Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis.Journal of clinical microbiology · 2009Article
- Geographic distribution and antifungal susceptibility of the newly described species Candida orthopsilosis and Candida metapsilosis in comparison to the closely related species Candida parapsilosis.Journal of clinical microbiology · 2008Article
- Molecular identification of Candida orthopsilosis isolated from blood culture.Mycopathologia · 2008Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Random amplified polymorphic DNA (RAPD) was used to better characterize the genotypic relatedness among medically important Candida species. By using short oligomer primers (10-mers) with arbitrarily chosen sequences in the polymerase chain reaction, distinctive and reproducible sets of polymerase chain reaction products were observed for isolates of C. albicans, C. lusitaniae, C. tropicalis, and Torulopsis (Candida) glabrata. The RAPD analysis differentiated a physiologically homogeneous panel of C. parapsilosis into three distinct groups and showed genetic diversity within C. haemulonii. Intraspecies DNA-length polymorphisms were seen for RAPD profiles derived from different isolates of each species. Analysis of RAPDs from a panel of C. albicans, which included 16 laboratory derivatives of two reference strains, showed that the profiles of unrelated strains differed and that the derivatives of each reference strain were identifiable. Minor differences in the RAPD profiles, suggestive of mutations that had occurred during the long-term maintenance of the strains, were detected. Because of its ease and reliability, RAPD analysis should be useful in providing genotypic characters for taxonomic descriptions, for confirming the identities of stock isolates, for typing Candida species in epidemiologic investigations, and for use in the rapid identification of pathogenic fungi.
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