ArticlePlant physiology2003
Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria digitata.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 98 citations in OpenAlex.
- Mode of Action of AlgE1: A Modular Mannuronate C-5 Epimerase.Biochemistry · 2025Article
- Candidate genes involved in biosynthesis and degradation of the main extracellular matrix polysaccharides of brown algae and their probable evolutionary history.BMC genomics · 2024Article
- Characterization of Unfractionated Polysaccharides in Brown Seaweed by Methylation-GC-MS-Based Linkage Analysis.Marine drugs · 2024Article
- Identification of differential gene expression related to reproduction in the sporophytes ofFrontiers in plant science · 2024Article
- Naturally occurring beneficial bacteriamBio · 2023Article
- Mechanistic Basis for Understanding the Dual Activities of the Bifunctional Azotobacter vinelandii Mannuronan C-5-Epimerase and Alginate Lyase AlgE7.Applied and environmental microbiology · 2022Article
- Standard Candles for Dating Microbial Lineages.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Cell Wall Polysaccharides Biosynthesis in Seaweeds: A Molecular Perspective.Frontiers in plant science · 2022Review
- Review
- Alginates along the filament of the brown alga Ectocarpus help cells cope with stress.Scientific reports · 2019Article
- Production of Hexaric Acids from Biomass.International journal of molecular sciences · 2019Review
- Functional genomics analysis reveals the biosynthesis pathways of important cellular components (alginate and fucoidan) of Saccharina.Current genetics · 2018Article
- Egg activation-triggered shape change in the Dictyota dichotoma (Phaeophyceae) zygote is actin-myosin and secretion dependent.Annals of botany · 2017Article
- Article
- Comparative characterization of two GDP-mannose dehydrogenase genes from Saccharina japonica (Laminariales, Phaeophyceae).BMC plant biology · 2016Article
- Microbiota Influences Morphology and Reproduction of the Brown Alga Ectocarpus sp.Frontiers in microbiology · 2016Article
- Insights into the structure and function of membrane-integrated processive glycosyltransferases.Current opinion in structural biology · 2015Review
- Saccharina genomes provide novel insight into kelp biology.Nature communications · 2015Article
- Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides.Chemical communications (Cambridge, England) · 2015Article
- Alginate-modifying enzymes: biological roles and biotechnological uses.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alginate is an industrially important polysaccharide obtained commercially by harvesting brown algae. The final step in alginate biosynthesis, the epimerization of beta-1,4-d-mannuronic acid to alpha-1,4-l-guluronic acid, a structural change that controls the physicochemical properties of the alginate, is catalyzed by the enzyme mannuronan C-5-epimerase. Six different cDNAs with homology to bacterial mannuronan C-5-epimerases were isolated from the brown alga Laminaria digitata (Phaeophyceae). Hydrophobic cluster analysis indicated that the proteins encoded by the L. digitata sequences have important structural similarities to the bacterial mannuronan C-5-epimerases, including conservation of the catalytic site. The expression of the C-5-epimerase genes was examined by northern-blot analysis and reverse transcriptase-polymerase chain reaction in L. digitata throughout a year. Expression was also monitored in protoplast cultures by northern and western blot, reverse transcriptase-polymerase chain reaction, and activity measurements. From both the structural comparisons and the expression pattern, it appears that the cDNAs isolated from L. digitata encode functional mannuronan C-5-epimerases. The phylogenetic relationships of the bacterial and brown algal enzymes and the inferences on the origin of alginate biosynthetic machinery are discussed.
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