ArticleJournal of bacteriology2003
An archaeal chromosomal autonomously replicating sequence element from an extreme halophile, Halobacterium sp. strain NRC-1.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 83 citations in OpenAlex.
- A virus-borne DNA damage signaling pathway controls the lysogeny-induction switch in a group of temperate pleolipoviruses.Nucleic acids research · 2023Article
- Origins of DNA replication.PLoS genetics · 2019Review
- Characterization of Copy Number Control of Two Haloferax volcanii Replication Origins Using Deletion Mutants and Haloarchaeal Artificial Chromosomes.Journal of bacteriology · 2018Article
- Construction of Expression Shuttle Vectors for the HaloarchaeonArchaea (Vancouver, B.C.) · 2017Article
- Archaeal extrachromosomal genetic elements.Microbiology and molecular biology reviews : MMBR · 2015Review
- Choosing a suitable method for the identification of replication origins in microbial genomes.Frontiers in microbiology · 2015Review
- Association between the dynamics of multiple replication origins and the evolution of multireplicon genome architecture in haloarchaea.Genome biology and evolution · 2014Article
- Recent Advances in the Identification of Replication Origins Based on the Z-curve Method.Current genomics · 2014Article
- Multiple replication origins with diverse control mechanisms in Haloarcula hispanica.Nucleic acids research · 2014Article
- Characterization of the replication initiator Orc1/Cdc6 from the Archaeon Picrophilus torridus.Journal of bacteriology · 2014Article
- DNA replication origins in archaea.Frontiers in microbiology · 2014Review
- The cell cycle of archaea.Nature reviews. Microbiology · 2013Review
- DoriC 5.0: an updated database of oriC regions in both bacterial and archaeal genomes.Nucleic acids research · 2013Article
- Diversity and evolution of multiple orc/cdc6-adjacent replication origins in haloarchaea.BMC genomics · 2012Article
- DNA replication and strand asymmetry in prokaryotic and mitochondrial genomes.Current genomics · 2012Article
- The origin recognition complex: a biochemical and structural view.Sub-cellular biochemistry · 2012Review
- Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.Applied and environmental microbiology · 2011Article
- Evolutionary rates and gene dispensability associate with replication timing in the archaeon Sulfolobus islandicus.Genome biology and evolution · 2010Article
- Quantitative analysis of replication-related mutation and selection pressures in bacterial chromosomes and plasmids using generalised GC skew index.BMC genomics · 2009Article
- Multiple replication origins of Halobacterium sp. strain NRC-1: properties of the conserved orc7-dependent oriC1.Journal of bacteriology · 2009Article
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2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on the identification and first cloning of an autonomously replicating sequence element from the chromosome of an archaeon, the extreme halophile Halobacterium strain NRC-1. The putative replication origin was identified by association with the orc7 gene and replication ability in the host strain, demonstrated by cloning into a nonreplicating plasmid. Deletion analysis showed that sequences located up to 750 bp upstream of the orc7 gene translational start, plus the orc7 gene and 50 bp downstream, are sufficient to endow the plasmid with replication ability, as judged by expression of a plasmid-encoded mevinolin resistance selectable marker and plasmid recovery after transformation. Sequences located proximal to the two other chromosomally carried haloarchaeal orc genes (orc6 and orc8) are not able to promote efficient autonomous replication. Located within the 750-bp region upstream of orc7 is a nearly perfect inverted repeat of 31 bp, which flanks an extremely AT-rich (44%) stretch of 189 bp. The replication ability of the plasmid was lost when one copy of the inverted repeat was deleted. Additionally, the inverted repeat structure near orc7 homologs in the genomic sequences of two other halophiles, Haloarcula marismortui and Haloferax volcanii, is highly conserved. Our results indicate that, in halophilic archaea, a chromosomal origin of replication is physically linked to orc7 homologs and that this element is sufficient to promote autonomous replication. We discuss the finding of a functional haloarchaeal origin in relation to the large number of orc1-cdc6 homologs identified in the genomes of all haloarchaea to date.
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