ArticlePlanta2004
cDNA cloning and expression of isoflavonoid-specific glucosyltransferase from Glycyrrhiza echinata cell-suspension cultures.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 43 citations in OpenAlex.
- Protein Modelling Highlighted Key Catalytic Sites Involved in Position-Specific Glycosylation of Isoflavonoids.International journal of molecular sciences · 2023Article
- Glycosylation and methylation in the biosynthesis of isoflavonoids inFrontiers in plant science · 2023Review
- Characterization of a heat responsive UDP: Flavonoid glucosyltransferase gene in tea plant (Camellia sinensis).PloS one · 2018Article
- Global Transcriptome Analyses Reveal Differentially Expressed Genes of Six Organs and Putative Genes Involved in (Iso)flavonoid Biosynthesis inFrontiers in plant science · 2018Article
- When plants produce not enough or at all: metabolic engineering of flavonoids in microbial hosts.Frontiers in plant science · 2015Review
- Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata.Plant cell reports · 2014Article
- Structure, function, and engineering of enzymes in isoflavonoid biosynthesis.Functional & integrative genomics · 2011Review
- Article
- EST analysis reveals putative genes involved in glycyrrhizin biosynthesis.BMC genomics · 2010Article
- Local differentiation of sugar donor specificity of flavonoid glycosyltransferase in Lamiales.The Plant cell · 2009Article
- A functional genomics approach to (iso)flavonoid glycosylation in the model legume Medicago truncatula.Plant molecular biology · 2007Article
- Pathogen-responsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis.Plant physiology · 2005Article
- A class of plant glycosyltransferases involved in cellular homeostasis.The EMBO journal · 2004Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding UDP-glucose: formononetin 7- O-glucosyltransferase, designated UGT73F1, was cloned from yeast extract-treated Glycyrrhiza echinata L. cell-suspension cultures using probes from Scutellaria baicalensis UDP-glucose: flavonoid 7- O-glucosyltransferase. The open reading frame of the UGT73F1 cDNA encodes a 441-amino-acid protein with a predicted molecular mass of 48.7 kDa. The deduced amino acid sequence showed that the protein is related to the stress-inducible glucosyltransferases. UGT73F1 mRNA was not detected in untreated G. echinata cultures but was transiently induced by treatment with yeast extract. Recombinant UGT73F1 was expressed as a histidine-tag fusion protein in Escherichia coli and purified to near homogeneity by nickel chelate chromatography. The purified recombinant enzyme was selective for isoflavonoid, formononetin and daidzein as substrates, while flavonoids and various tested non-flavonoid compounds were poor substrates.
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