Evidence map›Paper›PMID 14523618›Full record

ArticleJournal of plant research2003

Cell-cycle-related variation in proteins in suspension-cultured rice cells.

Tomomasa Takase, Yuki Yanagawa, Setsuko Komatsu, Hiroki Nakagawa, Junji Hashimoto

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of plant research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Tomomasa TakaseDepartment of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan.
Yuki Yanagawa
Setsuko Komatsu
Hiroki Nakagawa
Junji Hashimoto
Chiba University · JPInstitute of Agrobiological Sciences · JPYale University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To understand the cell cycle process in plants, we searched for proteins that quantitatively change during the cell cycle in suspension-cultured rice ( Oryza sativa L.) cells. The proteins were analyzed by a two-dimensional polyacrylamide gel electrophoresis image-analysis system. We detected 11 proteins that quantitatively changed during the cell cycle, among which beta-tubulins and a calreticulin-like protein were identified. The amounts of beta-tubulin proteins were low in the M phase and high in the G1 phase. In contrast, mRNAs for two of the three types of beta-tubulin were high in the M phase of the cell cycle. The addition of protease inhibitors MG132 or E64d to the cells decreased the beta-tubulin proteins during 24 h, suggesting that beta-tubulin proteins are degraded in vivo by proteases other than those whose activities are inhibited by MG132 or E64d.

Indexed as

Amino Acid SequenceCell CycleCells, CulturedCycloheximideElectrophoresis, Gel, Two-DimensionalKineticsOryzaPeptide FragmentsPlant ProteinsCycloheximidePeptide FragmentsPlant Proteins

Identifiers

PMID14523618
OpenAlexW2150923220

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.