ArticleInfection and immunity1992
Yersinia pestis YopM: thrombin binding and overexpression.
Article in Infection and immunity, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Cholinergic System and Its Therapeutic Importance in Inflammation and Autoimmunity.Frontiers in immunology · 2021Review
- Immunomodulatory Yersinia outer proteins (Yops)-useful tools for bacteria and humans alike.Virulence · 2017Review
- Distribution and Evolution of Yersinia Leucine-Rich Repeat Proteins.Infection and immunity · 2016Article
- Yersinia type III effectors perturb host innate immune responses.World journal of biological chemistry · 2016Review
- Toward a molecular pathogenic pathway for Yersinia pestis YopM.Frontiers in cellular and infection microbiology · 2012Article
- Yersinia virulence factor YopM induces sustained RSK activation by interfering with dephosphorylation.PloS one · 2010Article
- Gr1+ cells control growth of YopM-negative yersinia pestis during systemic plague.Infection and immunity · 2009Article
- The plague virulence protein YopM targets the innate immune response by causing a global depletion of NK cells.Infection and immunity · 2004Article
- Characterization of the Xanthomonas axonopodis pv. glycines Hrp pathogenicity island.Journal of bacteriology · 2003Article
- Application of a Saccharomyces cerevisiae model to study requirements for trafficking of Yersinia pestis YopM in eucaryotic cells.Infection and immunity · 2003Article
- Role of Yops and adhesins in resistance of Yersinia enterocolitica to phagocytosis.Infection and immunity · 2002Article
- Yersinia pestis pFra shows biovar-specific differences and recent common ancestry with a Salmonella enterica serovar Typhi plasmid.Journal of bacteriology · 2001Article
- DNA sequence and comparison of virulence plasmids from Rhodococcus equi ATCC 33701 and 103.Infection and immunity · 2000Article
- Apically exposed, tight junction-associated beta1-integrins allow binding and YopE-mediated perturbation of epithelial barriers by wild-type Yersinia bacteria.Infection and immunity · 2000Article
- Immune response to Yersinia outer proteins and other Yersinia pestis antigens after experimental plague infection in mice.Infection and immunity · 1999Article
- Protective efficacy of recombinant Yersinia outer proteins against bubonic plague caused by encapsulated and nonencapsulated Yersinia pestis.Infection and immunity · 1999Article
- The virulence plasmid of Yersinia, an antihost genome.Microbiology and molecular biology reviews : MMBR · 1998Review
- Type III protein secretion systems in bacterial pathogens of animals and plants.Microbiology and molecular biology reviews : MMBR · 1998Review
- Effect of Yersinia pestis YopM on experimental plague.Infection and immunity · 1997Article
- Yersinia pestis--etiologic agent of plague.Clinical microbiology reviews · 1997Review
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2 authors.
Funding
Abstract
In previous studies, Yersinia pestis YopM has been shown through mutational analysis to be necessary for virulence in mice and found to have homology with the thrombin-binding domain of the platelet receptor GPIb alpha. In this study, YopM was purified and shown by dot blot and chemical cross-linking tests to bind to human alpha-thrombin. No cross-linked product could be detected when human prothrombin was incubated with YopM. As a functional test of thrombin binding, it was shown that native but not boiled YopM inhibits thrombin-induced aggregation of human platelets. Control tests showed that YopM did not inactivate the platelets themselves, nor was its effect a nonspecific consequence of its very acidic isoelectric point. Microsequencing of YopM revealed an intact N terminus, indicating that functional YopM is not processed at the N terminus or secreted by a mechanism involving a cleavable signal sequence. Further characterization was made of an interesting effect on yopM expression that had been noticed in a previous study. A 1.5-kb HaeIII subclone overexpressed YopM in both Y. pestis and Escherichia coli compared with a larger clone containing the 5.3-kb HindIII-F fragment. To search for a possible regulator of YopM expression, the HindIII-F fragment was sequenced, revealing several open reading frames and three large repeated sequences. Deletional analysis showed that these were not involved in regulation of yopM. The data implicated a DNA structure 5' to yopM in moderating yopM expression.
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