ArticleGenome biology2003
Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing.
Article in Genome biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Microarrays and the Dawn of Omics Data Analysis.Advances in experimental medicine and biology · 2026Review
- Identification of Functional SSR Markers in Freshwater Ornamental Shrimps Neocaridina denticulata Using Transcriptome Sequencing.Marine biotechnology (New York, N.Y.) · 2020Article
- MyoMiner: explore gene co-expression in normal and pathological muscle.BMC medical genomics · 2020Article
- Overview of DNA microarrays: types, applications, and their future.Current protocols in molecular biology · 2013Article
- Quantitative analysis of 5HT(2C) receptor RNA editing patterns in psychiatric disorders.Neurobiology of disease · 2012Review
- NSMAP: a method for spliced isoforms identification and quantification from RNA-Seq.BMC bioinformatics · 2011Article
- Global profiling and molecular characterization of alternative splicing events misregulated in lung cancer.Molecular and cellular biology · 2011Article
- Global regulation of alternative splicing during myogenic differentiation.Nucleic acids research · 2010Article
- High-throughput identification of genetic markers using representational oligonucleotide microarray analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Development of a novel splice array platform and its application in the identification of alternative splice variants in lung cancer.BMC genomics · 2010Article
- Definition, conservation and epigenetics of housekeeping and tissue-enriched genes.BMC genomics · 2009Article
- Use of gene expression profiles of peripheral blood lymphocytes to distinguish BRCA1 mutation carriers in high risk breast cancer families.Cancer informatics · 2009Article
- ACC2 is expressed at high levels in human white adipose and has an isoform with a novel N-terminus [corrected].PloS one · 2009Article
- Expression of 24,426 human alternative splicing events and predicted cis regulation in 48 tissues and cell lines.Nature genetics · 2008Article
- Exon and junction microarrays detect widespread mouse strain- and sex-bias expression differences.BMC genomics · 2008Article
- Alternative splicing regulation during C. elegans development: splicing factors as regulated targets.PLoS genetics · 2008Article
- ALEXA: a microarray design platform for alternative expression analysis.Nature methods · 2008Article
- SPACE: an algorithm to predict and quantify alternatively spliced isoforms using microarrays.Genome biology · 2008Article
- Mapping the genome landscape using tiling array technology.Current opinion in plant biology · 2007Review
- Collective properties of evolving molecular quasispecies.BMC evolutionary biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microarrays offer a high-resolution means for monitoring pre-mRNA splicing on a genomic scale. We have developed a novel, unbiased amplification protocol that permits labeling of entire transcripts. Also, hybridization conditions, probe characteristics, and analysis algorithms were optimized for detection of exons, exon-intron edges, and exon junctions. These optimized protocols can be used to detect small variations and isoform mixtures, map the tissue specificity of known human alternative isoforms, and provide a robust, scalable platform for high-throughput discovery of alternative splicing.
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Registered trials
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