ArticleMolecular biology of the cell2003
Actin polymerization in macrophages in response to oxidized LDL and apoptotic cells: role of 12/15-lipoxygenase and phosphoinositide 3-kinase.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
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- Free-radicals and advanced chemistries involved in cell membrane organization influence oxygen diffusion and pathology treatment.AIMS biophysics · 2017Article
- Oxidized LDL induces phosphorylation of non-muscle myosin IIA heavy chain in macrophages.BMB reports · 2015Article
- Alteration of actin dependent signaling pathways associated with membrane microdomains in hyperlipidemia.Proteome science · 2015Article
- WAVE1 mediates suppression of phagocytosis by phospholipid-derived DAMPs.The Journal of clinical investigation · 2013Article
- Free-radical polymer science structural cancer model: a review.Scientifica · 2013Review
- Dietary supplementation with two Lamiaceae herbs-(oregano and sage) modulates innate immunity parameters in Lumbricus terrestris.Pharmacognosy research · 2013Article
- Overexpression of actin-depolymerizing factor blocks oxidized low-density lipoprotein-induced mouse brain microvascular endothelial cell barrier dysfunction.Molecular and cellular biochemistry · 2012Article
- The SYK side of TLR4: signalling mechanisms in response to LPS and minimally oxidized LDL.British journal of pharmacology · 2012Review
- The role of oxysterols in control of endothelial stiffness.Journal of lipid research · 2012Article
- Monocyte and macrophage dynamics during atherogenesis.Arteriosclerosis, thrombosis, and vascular biology · 2011Review
- Deletion of ABCA1 and ABCG1 impairs macrophage migration because of increased Rac1 signaling.Circulation research · 2011Article
- Oxidation-specific epitopes are danger-associated molecular patterns recognized by pattern recognition receptors of innate immunity.Circulation research · 2011 · on this mapReview
- Nutrigenetic disruption of inflammation-resolution homeostasis and atherogenesis.Journal of nutrigenetics and nutrigenomics · 2011Article
- Atherogenic lipids and lipoproteins trigger CD36-TLR2-dependent apoptosis in macrophages undergoing endoplasmic reticulum stress.Cell metabolism · 2010Article
- Oxidized cholesteryl esters and phospholipids in zebrafish larvae fed a high cholesterol diet: macrophage binding and activation.The Journal of biological chemistry · 2010Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Formation of filamentous F-actin drives many cellular processes, including phagocytosis and cell spreading. We have recently reported that mouse macrophage 12/15-lipoxygenase (12/15-LO) activity promotes F-actin formation in filopodia during phagocytosis of apoptotic cells. Oxidized low-density lipoprotein (OxLDL) also stimulates robust F-actin formation and spreading of macrophages. However, unlike apoptotic cells, OxLDL did not cause specific translocation of 12/15-LO to the cell membrane, neither in macrophages nor in GFP-15LO-transfected COS-7 cells. Moreover, inhibition of 12/15-LO activity in macrophages by a specific inhibitor or by 12/15-LO gene disruption did not affect OxLDL-induced actin polymerization. Among LDL modifications modeling OxLDL, LDL modified by incubation with 15LO-overexpressing fibroblasts was as active in eliciting F-actin response as was OxLDL. This LDL modification is well known to produce minimally modified LDL (mmLDL), which is bioactive and carries lipid oxidation products similar to those produced by 12/15-LO catalysis. MmLDL activated phosphoinositide 3-kinase (PI3K), and PI3K inhibitors abolished mmLDL-induced macrophage spreading. We hypothesize that OxLDL and mmLDL may contribute oxidized lipids to the macrophage cell membrane and thereby mimic intracellular 12/15-LO activity, which leads to uncontrolled actin polymerization and dramatic cytoskeletal changes in macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.