ArticleMolecular biology of the cell2003
Yeast kinetochores do not stabilize Stu2p-dependent spindle microtubule dynamics.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 78 citations in OpenAlex.
- Stu2 is required for plus-end directed chromosome transport along microtubules during metaphase inmicroPublication biology · 2025Article
- Spindle architecture constrains karyotype evolution.Nature cell biology · 2024Article
- Spindle architecture constrains karyotype in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- Robust microtubule dynamics facilitate low-tension kinetochore detachment in metaphase.The Journal of cell biology · 2023Article
- Polymer models reveal how chromatin modification can modulate force at the kinetochore.Molecular biology of the cell · 2022Article
- The TOG protein Stu2 is regulated by acetylation.PLoS genetics · 2022Article
- Kre28-Spc105 interaction is essential for Spc105 loading at the kinetochore.Open biology · 2022Article
- Mps1 promotes poleward chromosome movements in meiotic prometaphase.Molecular biology of the cell · 2021Article
- Article
- Delineating the contribution of Spc105-bound PP1 to spindle checkpoint silencing and kinetochore microtubule attachment regulation.The Journal of cell biology · 2019Article
- Recruitment of the Ulp2 protease to the inner kinetochore prevents its hyper-sumoylation to ensure accurate chromosome segregation.PLoS genetics · 2019Article
- Kinetochore-associated Stu2 promotes chromosome biorientation in vivo.PLoS genetics · 2019Article
- The regulation of chromosome segregation via centromere loops.Critical reviews in biochemistry and molecular biology · 2019Review
- Checkpoint Proteins Bub1 and Bub3 Delay Anaphase Onset in Response to Low Tension Independent of Microtubule-Kinetochore Detachment.Cell reports · 2019Article
- Geometric partitioning of cohesin and condensin is a consequence of chromatin loops.Molecular biology of the cell · 2018Article
- A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation.Current biology : CB · 2018Article
- The TOG protein Stu2/XMAP215 interacts covalently and noncovalently with SUMO.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Stu2 acts as a microtubule destabilizer in metaphase budding yeast spindles.Molecular biology of the cell · 2018Article
- Stu2 uses a 15-nm parallel coiled coil for kinetochore localization and concomitant regulation of the mitotic spindle.Molecular biology of the cell · 2018Article
- Structural plasticity of the living kinetochore.The Journal of cell biology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The interaction of kinetochores with dynamic microtubules during mitosis is essential for proper centromere motility, congression to the metaphase plate, and subsequent anaphase chromosome segregation. Budding yeast has been critical in the discovery of proteins necessary for this interaction. However, the molecular mechanism for microtubule-kinetochore interactions remains poorly understood. Using live cell imaging and mutations affecting microtubule binding proteins and kinetochore function, we identify a regulatory mechanism for spindle microtubule dynamics involving Stu2p and the core kinetochore component, Ndc10p. Depleting cells of the microtubule binding protein Stu2p reduces kinetochore microtubule dynamics. Centromeres remain under tension but lack motility. Thus, normal microtubule dynamics are not required to maintain tension at the centromere. Loss of the kinetochore (ndc10-1, ndc10-2, and ctf13-30) does not drastically affect spindle microtubule turnover, indicating that Stu2p, not the kinetochore, is the foremost governor of microtubule dynamics. Disruption of kinetochore function with ndc10-1 does not affect the decrease in microtubule turnover in stu2 mutants, suggesting that the kinetochore is not required for microtubule stabilization. Remarkably, a partial kinetochore defect (ndc10-2) suppresses the decreased spindle microtubule turnover in the absence of Stu2p. These results indicate that Stu2p and Ndc10p differentially function in controlling kinetochore microtubule dynamics necessary for centromere movements.
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