ArticleMolecular biology of the cell2003
Localization of a class III myosin to filopodia tips in transfected HeLa cells requires an actin-binding site in its tail domain.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 59 citations in OpenAlex.
- Characterization of a novel MYO3A missense mutation associated with a dominant form of late onset hearing loss.Scientific reports · 2018Trial
- Exploring phosphoregulation of MYO3A using quantitative fluorescence image analysis in COS7 cells.microPublication biology · 2026Article
- Single-molecule fluorescence microscopy reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of live inner ear hair cells.Nature communications · 2025Article
- Myosin 15 participates in assembly and remodeling of the presynapse.The Journal of cell biology · 2025Article
- The Prevalence and Clinical Characteristics ofGenes · 2025Article
- Pathophysiology of human hearing loss associated with variants in myosins.Frontiers in physiology · 2024Review
- The actin cytoskeleton in hair bundle development and hearing loss.Hearing research · 2023Review
- Deafness mutation in the MYO3A motor domain impairs actin protrusion elongation mechanism.Molecular biology of the cell · 2022Article
- The many roles of myosins in filopodia, microvilli and stereocilia.Current biology : CB · 2021Review
- Functional Role of Class III Myosins in Hair Cells.Frontiers in cell and developmental biology · 2021Review
- Myosin-Driven Intracellular Transport.Cold Spring Harbor perspectives in biology · 2018Review
- Filopodia formation and endosome clustering induced by mutant plus-end-directed myosin VI.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Impact of the Motor and Tail Domains of Class III Myosins on Regulating the Formation and Elongation of Actin Protrusions.The Journal of biological chemistry · 2016Article
- A single-headed fission yeast myosin V transports actin in a tropomyosin-dependent manner.The Journal of cell biology · 2016Article
- Genomic screening of ABCA4 and array CGH analysis underline the genetic variability of Greek patients with inherited retinal diseases.Meta gene · 2016Article
- Article
- Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.The Journal of cell biology · 2016Article
- Invertebrate and vertebrate class III myosins interact with MORN repeat-containing adaptor proteins.PloS one · 2015Article
- Phosphorylation of the kinase domain regulates autophosphorylation of myosin IIIA and its translocation in microvilli.Biochemistry · 2014Article
- Myosin IIIB uses an actin-binding motif in its espin-1 cargo to reach the tips of actin protrusions.Current biology : CB · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Bass Myo3A, a class III myosin, was expressed in HeLa cells as a GFP fusion in order to study its cellular localization. GFP-Myo3A localized to the cytoplasm and to the tips of F-actin bundles in filopodia, a localization that is consistent with the observed concentration toward the distal ends of F-actin bundles in photoreceptor cells. A mutation in the motor active site resulted in a loss of filopodia localization, suggesting that Myo3A motor activity is required for filopodial tip localization. Deletion analyses showed that the NH2-terminal kinase domain is not required but the CO2H-terminal 22 amino acids of the Myo3A tail are required for filopodial localization. Expression of this tail fragment alone produced fluorescence associated with F-actin throughout the cytoplasm and filopodia and a recombinant tail fragment bound to F-actin in vitro. An actin-binding motif was identified within this tail fragment, and a mutation within this motif abolished both filopodia localization by Myo3A and F-actin binding by the tail fragment alone. Calmodulin localized to filopodial tips when coexpressed with Myo3A but not in the absence of Myo3A, an observation consistent with the previous proposal that class III myosins bind calmodulin and thereby localize it in certain cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.