ArticleMolecular and cellular biology2003
Nuclear Pnn/DRS protein binds to spliced mRNPs and participates in mRNA processing and export via interaction with RNPS1.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 68 citations in OpenAlex.
- Cold exposure stimulates cross-tissue metabolic rewiring to fuel glucose-dependent thermogenesis in brown adipose tissue.Science advances · 2025Article
- Identification of a Novel miR-195-5p/PNN Axis in Colorectal Cancer.International journal of molecular sciences · 2024Article
- A molecular brake that modulates spliceosome pausing at detained introns contributes to neurodegeneration.Protein & cell · 2023Article
- Exon Junction Complexes can have distinct functional flavours to regulate specific splicing events.Scientific reports · 2018Article
- Co-expression networks reveal the tissue-specific regulation of transcription and splicing.Genome research · 2017Article
- Review
- Multifaceted Regulation of Gene Expression by the Apoptosis- and Splicing-Associated Protein Complex and Its Components.International journal of biological sciences · 2017Review
- Pinin facilitated proliferation and metastasis of colorectal cancer through activating EGFR/ERK signaling pathway.Oncotarget · 2016Article
- The exon junction complex as a node of post-transcriptional networks.Nature reviews. Molecular cell biology · 2016Review
- Transcriptome-Wide Identification of RNA Targets of Arabidopsis SERINE/ARGININE-RICH45 Uncovers the Unexpected Roles of This RNA Binding Protein in RNA Processing.The Plant cell · 2015Article
- The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript.Genes & development · 2014Article
- The exon junction complex is required for definition and excision of neighboring introns in Drosophila.Genes & development · 2014Article
- The RNA-binding protein Y14 inhibits mRNA decapping and modulates processing body formation.Molecular biology of the cell · 2013Article
- RNA splicing: a new player in the DNA damage response.International journal of cell biology · 2013Review
- Dissection of the role of Pinin in the development of zebrafish posterior pharyngeal cartilages.Histochemistry and cell biology · 2012Article
- Recent progress in histochemistry and cell biology.Histochemistry and cell biology · 2012Review
- The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex.Nature structural & molecular biology · 2012Article
- Pnn and SR family proteins are differentially expressed in mouse central nervous system.Histochemistry and cell biology · 2011Article
- The exon junction complex component Y14 modulates the activity of the methylosome in biogenesis of spliceosomal small nuclear ribonucleoproteins.The Journal of biological chemistry · 2011Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pnn/DRS protein is associated with desmosomes and colocalizes with splicing factors in nuclear speckled domains. The potential interaction of Pnn with RNPS1, a pre-mRNA splicing factor and a component of the exon-exon junction complex, prompted us to examine whether Pnn is involved in nuclear mRNA processing. By immunoprecipitation, we found that Pnn associates preferentially with mRNAs produced by splicing in vitro. Oligonucleotide-directed RNase H digestion revealed that Pnn binds to the spliced mRNAs at a position immediately upstream of the splice junction and that 5' splice site utilization determines the location of Pnn in alternatively spliced mRNAs. Immunoprecipitation further showed that Pnn binds to mRNAs produced from a transiently expressed reporter in vivo. Although associated with mRNPs, Pnn is a nuclear-restricted protein as revealed by the heterokaryon assay. Overexpression of an amino-terminal fragment of Pnn that directly interacts with RNPS1 leads to blockage of pre-mRNA splicing. However, although suppression of Pnn expression shows no significant effect on splicing, it leads to some extent to nuclear accumulation of bulk poly(A)(+) RNA. Therefore, Pnn may participate, via its interaction with RNPS1, in mRNA metabolism in the nucleus, including mRNA splicing and export.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.