ArticleMolecular and cellular biology2003
Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Rewards of divergence in sequences, 3-D structures and dynamics of yeast and human spliceosome SF3b complexes.Current research in structural biology · 2021Article
- Human Cancer-Associated Mutations of SF3B1 Lead to a Splicing Modification of Its Own RNA.Cancers · 2020Article
- Structural and functional modularity of the U2 snRNP in pre-mRNA splicing.Critical reviews in biochemistry and molecular biology · 2019Review
- Structural and mechanistic insights into human splicing factor SF3b complex derived using an integrated approach guided by the cryo-EM density maps.RNA biology · 2016Article
- An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses.PLoS pathogens · 2015Article
- Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.Nature structural & molecular biology · 2014Article
- The pre-mRNA retention and splicing complex controls tRNA maturation by promoting TAN1 expression.Nucleic acids research · 2013Article
- Article
- RRW: repeated random walks on genome-scale protein networks for local cluster discovery.BMC bioinformatics · 2009Article
- Structure of the yeast Pml1 splicing factor and its integration into the RES complex.Nucleic acids research · 2009Article
- Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- A BBP-Mud2p heterodimer mediates branchpoint recognition and influences splicing substrate abundance in budding yeast.Nucleic acids research · 2008Article
- A fungal family of transcriptional regulators: the zinc cluster proteins.Microbiology and molecular biology reviews : MMBR · 2006Review
- A subset of Mer1p-dependent introns requires Bud13p for splicing activation and nuclear retention.RNA (New York, N.Y.) · 2006Article
- Autoregulation of the mRNA export factor Yra1p requires inefficient splicing of its pre-mRNA.RNA (New York, N.Y.) · 2006Article
- U2 snRNA-protein contacts in purified human 17S U2 snRNPs and in spliceosomal A and B complexes.Molecular and cellular biology · 2006Article
- Interactions of the yeast SF3b splicing factor.Molecular and cellular biology · 2005Article
- Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing.The EMBO journal · 2004Article
- Biochemical analysis of TREX complex recruitment to intronless and intron-containing yeast genes.The EMBO journal · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Rds3p is a well-conserved 12-kDa protein with five CxxC zinc fingers that has been implicated in the activation of certain drug transport genes and in the pre-mRNA splicing pathway. Here we show that Rds3p resides in the yeast spliceosome and is essential for splicing in vitro. Rds3p purified from yeast stably associates with at least five U2 snRNP proteins, Cus1p, Hsh49p, Hsh155p, Rse1p, and Ist3p/Snu17p, and with the Yra1p RNA export factor. A mutation upstream of the first Rds3p zinc finger causes the conditional release of the putative branchpoint nucleotide binding protein, Ist3p/Snu17p, and weakens Rse1p interaction with the Rds3p complex. The resultant U2 snRNP particle migrates exceptionally slowly in polyacrylamide gels, suggestive of a disorganized structure. U2 snRNPs depleted of Rds3p fail to form stable prespliceosomes, although U2 snRNA stability is not affected. Metabolic depletion of Yra1p blocks cell growth but not splicing, suggesting that Yra1p association with Rds3p relates to Yra1p's role in RNA trafficking. Together these data establish Rds3p as an essential component of the U2 snRNP SF3b complex and suggest a new link between the nuclear processes of pre-mRNA splicing and RNA export.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.