ArticleThe Journal of general physiology2003
Molecular coupling between voltage sensor and pore opening in the Arabidopsis inward rectifier K+ channel KAT1.
Article in The Journal of general physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Structure mirroring function: What's the 'matter' with the funny current?The Journal of physiology · 2026Review
- Towards a unified gating scheme for the CNBD ion channel family.The Journal of general physiology · 2026Review
- Guard cells on the adaxial and abaxial leaf surfaces use different compositions of potassium ion channels to drive light-induced stomatal opening.Nature plants · 2025Article
- Voltage sensors.Molecular pharmacology · 2025Review
- Fifty years of gating currents and channel gating.The Journal of general physiology · 2023Review
- External Cd2+ and protons activate the hyperpolarization-gated K+ channel KAT1 at the voltage sensor.The Journal of general physiology · 2021Article
- A folding reaction at the C-terminal domain drives temperature sensing in TRPM8 channels.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Bipolar switching by HCN voltage sensor underlies hyperpolarization activation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Evolution and Structural Characteristics of Plant Voltage-Gated KThe Plant cell · 2018Review
- Gating control and KPlant, cell & environment · 2018Article
- Outward Rectification of Voltage-Gated K+ Channels Evolved at Least Twice in Life History.PloS one · 2015Article
- Voltage-sensor transitions of the inward-rectifying K+ channel KAT1 indicate a latching mechanism biased by hydration within the voltage sensor.Plant physiology · 2014Article
- Biochemical and structural analysis of the hyperpolarization-activated K(+) channel MVP.Biochemistry · 2014Article
- Voltage sensor of ion channels and enzymes.Biophysical reviews · 2012Review
- Article
- Cytoplasmic domains and voltage-dependent potassium channel gating.Frontiers in pharmacology · 2012Article
- A minimal cysteine motif required to activate the SKOR K+ channel of Arabidopsis by the reactive oxygen species H2O2.The Journal of biological chemistry · 2010Article
- Insights into the salt tolerance mechanism in barley (Hordeum vulgare) from comparisons of cultivars that differ in salt sensitivity.Journal of plant research · 2010Article
- Chemical tools for K(+) channel biology.Biochemistry · 2009Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Animal and plant voltage-gated ion channels share a common architecture. They are made up of four subunits and the positive charges on helical S4 segments of the protein in animal K+ channels are the main voltage-sensing elements. The KAT1 channel cloned from Arabidopsis thaliana, despite its structural similarity to animal outward rectifier K+ channels is, however, an inward rectifier. Here we detected KAT1-gating currents due to the existence of an intrinsic voltage sensor in this channel. The measured gating currents evoked in response to hyperpolarizing voltage steps consist of a very fast (tau = 318 +/- 34 micros at -180 mV) and a slower component (4.5 +/- 0.5 ms at -180 mV) representing charge moved when most channels are closed. The observed gating currents precede in time the ionic currents and they are measurable at voltages (less than or equal to -60) at which the channel open probability is negligible ( approximately 10-4). These two observations, together with the fact that there is a delay in the onset of the ionic currents, indicate that gating charge transits between several closed states before the KAT1 channel opens. To gain insight into the molecular mechanisms that give rise to the gating currents and lead to channel opening, we probed external accessibility of S4 domain residues to methanethiosulfonate-ethyltrimethylammonium (MTSET) in both closed and open cysteine-substituted KAT1 channels. The results demonstrate that the putative voltage-sensing charges of S4 move inward when the KAT1 channels open.
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