ArticleThe EMBO journal2003
Clathrin self-assembly involves coordinated weak interactions favorable for cellular regulation.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Reframing clathrin-mediated viral entry: evidence standards, context dependence and host-directed vulnerabilities.Frontiers in cellular and infection microbiology · 2026Review
- Protein dimerization in 2D vs 3D: Geometric allostery enhances binding affinity.The Journal of chemical physics · 2025Article
- Integrating protein copy numbers with interaction networks to quantify stoichiometry in clathrin-mediated endocytosis.Scientific reports · 2022Article
- Large self-assembled clathrin lattices spontaneously disassemble without sufficient adaptor proteins.PLoS computational biology · 2022Article
- NERDSS: A Nonequilibrium Simulator for Multibody Self-Assembly at the Cellular Scale.Biophysical journal · 2020Article
- Dynamic instability of clathrin assembly provides proofreading control for endocytosis.The Journal of cell biology · 2019Article
- Modifying Membrane Morphology and Interactions with DNA Origami Clathrin-Mimic Networks.ACS nano · 2019Article
- Stoichiometric balance of protein copy numbers is measurable and functionally significant in a protein-protein interaction network for yeast endocytosis.PLoS computational biology · 2018Article
- Cytosolic proteins can exploit membrane localization to trigger functional assembly.PLoS computational biology · 2018Article
- Clathrin-coated vesicles from brain have small payloads: a cryo-electron tomographic study.Journal of structural biology · 2013Article
- Micellization model for the polymerization of clathrin baskets.The Journal of chemical physics · 2013Article
- A cost-benefit analysis of the physical mechanisms of membrane curvature.Nature cell biology · 2013Review
- Clathrin-mediated endocytosis and adaptor proteins.Acta naturae · 2013Article
- Unraveling protein-protein interactions in clathrin assemblies via atomic force spectroscopy.Methods (San Diego, Calif.) · 2013Article
- Systems biology and physical biology of clathrin-mediated endocytosis.Integrative biology : quantitative biosciences from nano to macro · 2011Review
- Asymmetry as the key to clathrin cage assembly.Biophysical journal · 2010Article
- Conformation switching of clathrin light chain regulates clathrin lattice assembly.Developmental cell · 2010Article
- Monomeric but not trimeric clathrin heavy chain regulates p53-mediated transcription.Oncogene · 2008Article
- A geometric principle may guide self-assembly of fullerene cages from clathrin triskelia and from carbon atoms.Biophysical journal · 2008Article
- Allosteric signaling and a nuclear exit strategy: binding of UL25/UL17 heterodimers to DNA-Filled HSV-1 capsids.Molecular cell · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The clathrin triskelion self-assembles into a polyhedral coat surrounding membrane vesicles that sort receptor cargo to the endocytic pathway. A triskelion comprises three clathrin heavy chains joined at their C-termini, extending into proximal and distal leg segments ending in a globular N-terminal domain. In the clathrin coat, leg segments entwine into parallel and anti-parallel interactions. Here we define the contributions of segmental interactions to the clathrin assembly reaction and measure the strength of their interactions. Proximal and distal leg segments were found to lack sufficient affinity to form stable homo- or heterodimers under assembly conditions. However, chimeric constructs of proximal or distal leg segments, trimerized by replacement of the clathrin trimerization domain with that of the invariant chain protein, were able to self-assemble in reversible reactions. Thus clathrin assembly occurs because weak leg segment affinities are coordinated through trimerization, sharing a dependence on multiple weak interactions with other biopolymers. Such polymerization is sensitive to small environmental changes and is therefore compatible with cellular regulation of assembly, disassembly and curvature during formation of clathrin-coated vesicles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.