ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2003
Okadaic acid, an inhibitor of protein phosphatase 1 and 2A, induces premature separation of sister chromatids during meiosis I and aneuploidy in mouse oocytes in vitro.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 54 citations in OpenAlex.
- Oscillations in PP1 activity are essential for accurate progression through mammalian oocyte meiosis.Cell cycle (Georgetown, Tex.) · 2023Article
- Review
- Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis II.The EMBO journal · 2021Article
- Protein Phosphatase 2A B'α and B'β Protect Centromeric Cohesion during Meiosis I.Plant physiology · 2019Article
- A high glucose diet induces autophagy in a HLH-30/TFEB-dependent manner and impairs the normal lifespan ofAging · 2018Article
- PP2A regulates kinetochore-microtubule attachment during meiosis I in oocyte.Cell cycle (Georgetown, Tex.) · 2016Article
- Unique subcellular distribution of phosphorylated Plk1 (Ser137 and Thr210) in mouse oocytes during meiotic division and pPlk1(Ser137) involvement in spindle formation and REC8 cleavage.Cell cycle (Georgetown, Tex.) · 2015Article
- Sister chromatid segregation in meiosis II: deprotection through phosphorylation.Cell cycle (Georgetown, Tex.) · 2013Review
- SGO1 maintains bovine meiotic and mitotic centromeric cohesions of sister chromatids and directly affects embryo development.PloS one · 2013Article
- The role of shugoshin in meiotic chromosome segregation.Cytogenetic and genome research · 2011Review
- Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division.The EMBO journal · 2009Article
- Studies of meiosis disclose distinct roles of cohesion in the core centromere and pericentromeric regions.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Review
- Solving the shugoshin puzzle.Trends in genetics : TIG · 2008Review
- Regulation of spindle and chromatin dynamics during early and late stages of oocyte maturation by aurora kinases.Molecular human reproduction · 2008Article
- Functional interaction of Aurora-A and PP2A during mitosis.Molecular biology of the cell · 2007Article
- New insight into the role of phosphodiesterase 3A in porcine oocyte maturation.BMC developmental biology · 2006Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in understanding some of the molecular aspects of chromosome segregation during mitosis and meiosis provide a background for investigating potential mechanisms of aneuploidy in mammalian germ cells. Numerous protein kinases and phosphatases have important functions during mitosis and meiosis. Alterations in these enzyme activities may upset the normal temporal sequence of biochemical reactions and cellular organelle modifications required for orderly chromosome segregation. Protein phosphatases 1 (PP1) and 2A (PP2A) play integral roles in regulating oocyte maturation (OM) and the metaphase-anaphase transitions. Mouse oocytes were transiently exposed in vitro to different dosages (0, 0.01, 0.1, or 1.0 microg/ml) of the PP1 and PP2A phosphatase inhibitor okadaic acid (OA) during meiosis I and oocytes were cytogenetically analyzed. Significant (p < 0.05) OA dose-response increases in the frequencies of metaphase I (MI) arrested oocytes, MI oocytes with 80 chromatids instead of the normal 20 tetrads, and anaphase I telophase I (AI-TI) oocytes with two groups of an unequal number of chromatids were found. Analysis of MII oocytes revealed significant (p < 0.05) increases in the frequencies of premature sister chromatid separation, single-unpaired chromatids, and hyperploidy. Besides showing that OA is aneugenic, these data suggest that OA-induced protein phosphatase inhibition upsets the normal kinase-phosphatase equilibrium during mouse OM, resulting in precocious removal of cohesion proteins from chromosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.