ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2003
Immunogold labeling of chromosomes for scanning electron microscopy: a closer look at phosphorylated histone H3 in mitotic metaphase chromosomes of Hordeum vulgare.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Allyl-Isothiocyanate and Microcystin-LR Reveal the Protein Phosphatase Mediated Regulation of Metaphase-Anaphase Transition inFrontiers in plant science · 2018Article
- Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAScientific reports · 2017Article
- Low-voltage scanning electron microscopy study of lampbrush chromosomes and nuclear bodies in avian and amphibian oocytes.Scientific reports · 2016Article
- Article
- Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells.Plant cell reports · 2014Article
- Scanning electron microscope studies of human metaphase chromosomes.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2014Article
- Microcystin-LR and cylindrospermopsin induced alterations in chromatin organization of plant cells.Marine drugs · 2013Review
- Distribution pattern of histone H3 phosphorylation at serine 10 during mitosis and meiosis in Brachiaria species.Journal of genetics · 2013Article
- Microcystin-LR, a protein phosphatase inhibitor, induces alterations in mitotic chromatin and microtubule organization leading to the formation of micronuclei in Vicia faba.Annals of botany · 2012Article
- Visualization of gold and platinum nanoparticles interacting with Salmonella enteritidis and Listeria monocytogenes.International journal of nanomedicine · 2010Article
- CENH3 interacts with the centromeric retrotransposon cereba and GC-rich satellites and locates to centromeric substructures in barley.Chromosoma · 2007Article
- Quantum dots--a versatile tool in plant science?Journal of nanobiotechnology · 2006Article
- High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy.Chromosoma · 2006Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution detection of phosphorylated histone H3 at serine 10 in mitotic barley chromosomes for scanning electron microscopy was shown using a novel application of indirect immunogold labeling with Nanogold. This method permits localization and quantification of signals in a three-dimensional context. Because the chromosome structure is well preserved, characterization of binding sites (chromomeres, parallel matrix fibers, solenoids), currently in the realm of nanometer decades, is possible. Quantification and three-dimensional localization of labels is possible with stereoscopic analysis. Limitations of the method pertain to the challenges in preservation of chromosome ultrastructure, accessibility of immunoreactants into the fixed chromatin and unspecific labeling. The differences between silver and gold enhancement and the current status of labeling efficiency are addressed.
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