ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2003
Transpositional behaviour of an Ac/Ds system for reverse genetics in rice.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- Harnessing neo-domestication of wild pigmented rice for enhanced nutrition and sustainable agriculture.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Development of Ac- and Ds-tagged starter lines for large-scale transposon-mutagenesis in tomato.PloS one · 2025Article
- Establishment ofBreeding science · 2019Article
- A collection of enhancer trap insertional mutants for functional genomics in tomato.Plant biotechnology journal · 2017Article
- Transposon based activation tagging in diploid strawberry and monoploid derivatives of potato.Plant cell reports · 2014Article
- International Consortium of Rice Mutagenesis: resources and beyond.Rice (New York, N.Y.) · 2013Article
- A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Mutagenesis of barley malting quality QTLs with Ds transposons.Functional & integrative genomics · 2012Article
- Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery.BMC genomics · 2012Article
- Natural and artificial mutants as valuable resources for functional genomics and molecular breeding.International journal of biological sciences · 2010Review
- Genome-wide analysis of Tol2 transposon reintegration in zebrafish.BMC genomics · 2009Article
- Mutant resources in rice for functional genomics of the grasses.Plant physiology · 2009Article
- Analysis of gene-trap Ds rice populations in Korea.Plant molecular biology · 2007Article
- Generating novel allelic variation through Activator insertional mutagenesis in maize.Genetics · 2007Article
- A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes.Plant molecular biology · 2007Article
- High-frequency Ds remobilization over multiple generations in barley facilitates gene tagging in large genome cereals.Plant molecular biology · 2006Article
- Article
- EU-OSTID: a collection of transposon insertional mutants for functional genomics in rice.Plant molecular biology · 2005Article
- Reverse genetic approaches for functional genomics of rice.Plant molecular biology · 2005Review
- Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics.Plant molecular biology · 2005Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A collection of transposon Ac/ Ds enhancer trap lines is being developed in rice that will contribute to the development of a rice mutation machine for the functional analysis of rice genes. Molecular analyses revealed high transpositional activity in early generations, with 62% of the T0 primary transformants and more than 90% of their T1 progeny lines showing ongoing active transposition. About 10% of the lines displayed amplification of the Ds copy number. However, inactivation of Ds seemed to occur in about 70% of the T2 families and in the T3 generation. Southern blot analyses revealed a high frequency of germinal insertions inherited in the T1 progeny plants, and transmitted preferentially over the many other somatic inserts to later generations. The sequencing of Ds flanking sites in subsets of T1 plants indicated the independence of insertions in different T1 families originating from the same T0 line. Almost 80% of the insertion sites isolated showing homology to the sequenced genome, resided in genes or within a range at which neighbouring genes could be revealed by enhancer trapping. A strategy involving the propagation of a large number of T0 and T1 independent lines is being pursued to ensure the recovery of a maximum number of independent insertions in later generations. The inactive T2 and T3 lines produced will then provide a collection of stable insertions to be used in reverse genetics experiments. The preferential insertion of Ds in gene-rich regions and the use of lines containing multiple Ds transposons will enable the production of a large population of inserts in a smaller number of plants. Additional features provided by the presence of lox sites for site-specific recombination, or the use of different transposase sources and selectable markers, are discussed.
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