ArticleJournal of biomolecular NMR2003
Solid state NMR sequential resonance assignments and conformational analysis of the 2x10.4 kDa dimeric form of the Bacillus subtilis protein Crh.
Article in Journal of biomolecular NMR, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
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- Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.Quarterly reviews of biophysics · 2017Review
- Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization.Crystal growth & design · 2016Article
- Protein residue linking in a single spectrum for magic-angle spinning NMR assignment.Journal of biomolecular NMR · 2015Article
- Rapid proton-detected NMR assignment for proteins with fast magic angle spinning.Journal of the American Chemical Society · 2014Article
- Secondary structure in the core of amyloid fibrils formed from human β₂m and its truncated variant ΔN6.Journal of the American Chemical Society · 2014Article
- Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.PloS one · 2014Article
- Resonance assignment of the NMR spectra of disordered proteins using a multi-objective non-dominated sorting genetic algorithm.Journal of biomolecular NMR · 2013Article
- Secondary structural analysis of proteins based on (13)C chemical shift assignments in unresolved solid-state NMR spectra enhanced by fragmented structure database.Journal of biomolecular NMR · 2013Article
- Membrane protein structure determination: back to the membrane.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Mutant protein A30P α-synuclein adopts wild-type fibril structure, despite slower fibrillation kinetics.The Journal of biological chemistry · 2012Article
- Extensive de novo solid-state NMR assignments of the 33 kDa C-terminal domain of the Ure2 prion.Journal of biomolecular NMR · 2011Article
- Heteronuclear proton assisted recoupling.The Journal of chemical physics · 2011Article
- Resonance Assignments and Secondary Structure Analysis of Dynein Light Chain 8 by Magic Angle Spinning NMR Spectroscopy.Canadian journal of chemistry · 2011Article
- GFT projection NMR spectroscopy for proteins in the solid state.Journal of biomolecular NMR · 2010Article
- Automated protein resonance assignments of magic angle spinning solid-state NMR spectra of β1 immunoglobulin binding domain of protein G (GB1).Journal of biomolecular NMR · 2010Article
- High-resolution MAS NMR analysis of PI3-SH3 amyloid fibrils: backbone conformation and implications for protofilament assembly and structure .Biochemistry · 2010Article
- Assignment strategies for large proteins by magic-angle spinning NMR: the 21-kDa disulfide-bond-forming enzyme DsbA.Journal of molecular biology · 2010Article
- NMR Determination of Protein pK(a) Values in the Solid State.The journal of physical chemistry letters · 2010Article
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Authors and funding
10 authors.
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Abstract
Solid state NMR sample preparation and resonance assignments of the U-[13C,15N] 2x10.4 kDa dimeric form of the regulatory protein Crh in microcrystalline, PEG precipitated form are presented. Intra- and interresidue correlations using dipolar polarization transfer methods led to nearly complete sequential assignments of the protein, and to 88% of all 15N, 13C chemical shifts. For several residues, the resonance assignments differ significantly from those reported for the monomeric form analyzed by solution state NMR. Dihedral angles obtained from a TALOS-based statistical analysis suggest that the microcrystalline arrangement of Crh must be similar to the domain-swapped dimeric structure of a single crystal form recently solved using X-ray crystallography. For a limited number of protein residues, a remarkable doubling of the observed NMR resonances is observed indicative of local static or dynamic conformational disorder. Our study reports resonance assignments for the largest protein investigated by solid state NMR so far and describes the conformational dimeric variant of Crh with previously unknown chemical shifts.
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