ArticleJournal of virology2003
Tomato mosaic virus replication protein suppresses virus-targeted posttranscriptional gene silencing.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
67 citing papers in PubMed, 233 citations in OpenAlex.
- Tobamoviruses: Advances in Molecular Biology, Host Interactions and Integrated Disease Management.Biology · 2026Review
- A Tradeoff Between the Escape fromPlants (Basel, Switzerland) · 2025Article
- Tobacco Mosaic Virus Movement: From Capsid Disassembly to Transport Through Plasmodesmata.Viruses · 2025Review
- The roles of movement and coat proteins in the transport of tobamoviruses between plant cells.Frontiers in plant science · 2025Review
- Zinc gluconate protects against plant virus infection in tomato andPlant biotechnology (Tokyo, Japan) · 2024Article
- A novel ilarvirus protein CP-RT is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing.PLoS pathogens · 2024Article
- Molecular characterization and host reaction to tomato mottle mosaic virus isolated from sweet pepper seeds in Japan.Archives of virology · 2024Article
- Molecular Characterization and Pathogenicity of an Infectious cDNA Clone of Youcai Mosaic Virus onInternational journal of molecular sciences · 2024Article
- Article
- dsRNA-induced immunity targets plasmodesmata and is suppressed by viral movement proteins.The Plant cell · 2023Article
- Role of Plant Virus Movement Proteins in Suppression of Host RNAi Defense.International journal of molecular sciences · 2023Review
- Movement Protein Mediates Systemic Necrosis in Tomato Plants with Infection of Tomato Mosaic Virus.Viruses · 2023Article
- Article
- Identification of an anti-plant-virus molecule in Alpinia zerumbet.Bioresources and bioprocessing · 2021Article
- Turnip mosaic virus in oilseed rape activates networks of sRNA-mediated interactions between viral and host genomes.Communications biology · 2020Article
- Tomato Brown Rugose Fruit Virus Contributes to Enhanced Pepino Mosaic Virus Titers in Tomato Plants.Viruses · 2020Article
- A single amino acid in coat protein of Pepper mild mottle virus determines its subcellular localization and the chlorosis symptom on leaves of pepper.The Journal of general virology · 2020Article
- Inhibitory effects ofPlant biotechnology (Tokyo, Japan) · 2020Article
- Facilitative and synergistic interactions between fungal and plant viruses.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Synonymous SNPs of viral genes facilitate virus to escape host antiviral RNAi immunity.RNA biology · 2019Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Posttranscriptional gene silencing (PTGS), a homology-dependent RNA degradation system, has a role in defending against virus infection in plants, but plant viruses encode a suppressor to combat PTGS. Using transgenic tobacco in which the expression of green fluorescent protein (GFP) is posttranscriptionally silenced, we investigated a tomato mosaic virus (ToMV)-encoded PTGS suppressor. Infection with wild-type ToMV (L strain) interrupted GFP silencing in tobacco, coincident with visible symptoms, whereas some attenuated strains of ToMV (L(11) and L(11)A strains) failed to suppress GFP silencing. Analyses of recombinant viruses containing the L and L(11)A strains revealed that a single base change in the replicase gene, which causes an amino acid substitution, is responsible for the symptomless and suppressor-defective phenotypes of the attenuated strains. An agroinfiltration assay indicated that the 130K replication protein acts as a PTGS suppressor. Small interfering RNAs (siRNAs) of 21 to 25 nucleotides accumulated during ToMV infection, suggesting that the major target of the ToMV-encoded suppressor is downstream from the production of siRNAs in the PTGS pathway. Analysis with GFP-tagged recombinant viruses revealed that the suppressor inhibits the establishment of the ToMV-targeted PTGS system in the inoculated leaves but does not detectably suppress the activity of the preexisting, sequence-specific PTGS machinery there. Taken together, these results indicate that it is likely that the ToMV-encoded suppressor, the 130K replication protein, blocks the utilization of silencing-associated small RNAs, so that a homology-dependent RNA degradation machinery is not newly formed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.