ArticleThe Plant cell2003
The transport of prolamine RNAs to prolamine protein bodies in living rice endosperm cells.
Article in The Plant cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 81 citations in OpenAlex.
- Live Single-Cell Transcriptional Dynamics in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants.Plants (Basel, Switzerland) · 2024Review
- mRNA Localization to the Endoplasmic Reticulum in Plant Endosperm Cells.International journal of molecular sciences · 2022Review
- Hitchhiking Across Kingdoms: Cotransport of Cargos in Fungal, Animal, and Plant Cells.Annual review of cell and developmental biology · 2022Review
- Distinct Mechanisms of Endomembrane Reorganization Determine Dissimilar Transport Pathways in Plant RNA Viruses.Plants (Basel, Switzerland) · 2022Review
- Development of a split fluorescent protein-based RNA live-cell imaging system to visualize mRNA distribution in plants.Plant methods · 2022Article
- Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s.Scientific reports · 2020Article
- mRNA Localization in Plant Cells.Plant physiology · 2020Review
- Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant.Journal of experimental botany · 2018Article
- RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells.The Plant cell · 2018Article
- Selective Targeting of Mobile mRNAs to Plasmodesmata for Cell-to-Cell Movement.Plant physiology · 2018Article
- A Plant Biologist's Toolbox to Study Translation.Frontiers in plant science · 2018Review
- Multifunctional RNA Binding Protein OsTudor-SN in Storage Protein mRNA Transport and Localization.Plant physiology · 2017Article
- Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.Journal of experimental botany · 2016Article
- Protein Bodies in Leaves Exchange Contents through the Endoplasmic Reticulum.Frontiers in plant science · 2016Article
- Characterization of RNA binding protein RBP-P reveals a possible role in rice glutelin gene expression and RNA localization.Plant molecular biology · 2014Article
- Subcellular mRNA localisation at a glance.Journal of cell science · 2014Review
- mRNA localization to P-bodies in yeast is bi-phasic with many mRNAs captured in a late Bfr1p-dependent wave.Journal of cell science · 2014Article
- Multiple RNA binding protein complexes interact with the rice prolamine RNA cis-localization zipcode sequences.Plant physiology · 2014Article
- Translational Regulation of Cytoplasmic mRNAs.The arabidopsis book · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNAs that code for the major rice storage proteins are localized to specific subdomains of the cortical endoplasmic reticulum (ER) in developing endosperm. Prolamine RNAs are localized to the ER and delimit the prolamine intracisternal inclusion granules (PB-ER), whereas glutelin RNAs are targeted to the cisternal ER. To study the transport of prolamine RNAs to the surface of the prolamine protein bodies in living endosperm cells, we adapted a two-gene system consisting of green fluorescent protein (GFP) fused to the viral RNA binding protein MS2 and a hybrid prolamine RNA containing tandem MS2 RNA binding sites. Using laser scanning confocal microscopy, we show that the GFP-labeled prolamine RNAs are transported as particles that move at an average speed of 0.3 to 0.4 microm/s. These prolamine RNA transport particles generally move unidirectionally in a stop-and-go manner, although nonlinear bidirectional, restricted, and nearly random movement patterns also were observed. Transport is dependent on intact microfilaments, because particle movement is inhibited rapidly by the actin filament-disrupting drugs cytochalasin D and latrunculin B. Direct evidence was obtained that these prolamine RNA-containing particles are transported to the prolamine protein bodies. The significance of these results with regard to protein synthesis in plants is discussed.
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