ArticleThe Plant cell2003
Phospholipase d activation correlates with microtubule reorganization in living plant cells.
Article in The Plant cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 241 citations in OpenAlex.
- Xanthan Oligosaccharide Seed Coating Promotes Wheat Growth and Stress-Related Physiological Reprogramming via Hormone Signaling and Plant-Microbe Associations.Plants (Basel, Switzerland) · 2026Article
- Phospholipid Networks as Metabolic Hubs and Signaling Integrators in Plant Development and Stress Adaptation.Plants (Basel, Switzerland) · 2026Review
- Transcriptome analysis of wild soybean (Glycine soja) under salt stress and identification of salt-responsive genes.Genes & genomics · 2025Article
- Abscisic Acid, Microtubules and Phospholipase D-Solving a Cellular Bermuda Triangle.International journal of molecular sciences · 2024Article
- Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits byFoods (Basel, Switzerland) · 2023Article
- The cell biology of primary cell walls during salt stress.The Plant cell · 2023Review
- Phospholipase Dδ and phosphatidic acid mediate heat-induced nuclear localization of glyceraldehyde-3-phosphate dehydrogenase in Arabidopsis.The Plant journal : for cell and molecular biology · 2022Article
- Mechanical stress effects on transcriptional regulation of genes encoding microtubule- and actin-associated proteins.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat.BMC plant biology · 2021Article
- The Reduced Longitudinal Growth Induced by Overexpression ofPlants (Basel, Switzerland) · 2021Article
- Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase.Biology · 2020Article
- The Serine Carboxypeptidase-Like GenePlants (Basel, Switzerland) · 2020Article
- The BIR2/BIR3-Associated Phospholipase Dγ1 Negatively Regulates Plant Immunity.Plant physiology · 2020Article
- AtKATANIN1 Modulates Microtubule Depolymerization and Reorganization in Response to Salt Stress inInternational journal of molecular sciences · 2019Article
- Recent Advances in the Cellular and Developmental Biology of Phospholipases in Plants.Frontiers in plant science · 2019Review
- Shot-Gun Proteomic Analysis on Roots of ArabidopsisInternational journal of molecular sciences · 2018Article
- Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination inPlant diversity · 2018Article
- A Cycloheximide-Sensitive Step in Transverse Microtubule Array Patterning.Plant physiology · 2018Article
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26 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A phospholipase D (PLD) was shown recently to decorate microtubules in plant cells. Therefore, we used tobacco BY-2 cells expressing the microtubule reporter GFP-MAP4 to test whether PLD activation affects the organization of plant microtubules. Within 30 min of adding n-butanol, a potent activator of PLD, cortical microtubules were released from the plasma membrane and partially depolymerized, as visualized with four-dimensional confocal imaging. The isomers sec- and tert-butanol, which did not activate PLD, did not affect microtubule organization. The effect of treatment on PLD activation was monitored by the in vivo formation of phosphatidylbutanol, a specific reporter of PLD activity. Tobacco cells also were treated with mastoparan, xylanase, NaCl, and hypoosmotic stress as reported activators of PLD. We confirmed the reports and found that all treatments induced microtubule reorganization and PLD activation within the same time frame. PLD still was activated in microtubule-stabilized (taxol) and microtubule-depolymerized (oryzalin) situations, suggesting that PLD activation triggers microtubular reorganization and not vice versa. Exogenously applied water-soluble synthetic phosphatidic acid did not affect the microtubular cytoskeleton. Cell cycle studies revealed that n-butanol influenced not just interphase cortical microtubules but also those in the preprophase band and phragmoplast, but not those in the spindle structure. Cell growth and division were inhibited in the presence of n-butanol, whereas sec- and tert-butanol had no such effects. Using these novel insights, we propose a model for the mechanism by which PLD activation triggers microtubule reorganization in plant cells.
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