ArticleBiophysical journal2003
Adhesively-tensed cell membranes: lysis kinetics and atomic force microscopy probing.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 187 citations in OpenAlex.
- Chromatin decompaction within the nucleus increases plasma membrane tension promoting NETosis execution, independently of transcription.Nature communications · 2026Article
- Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter.bioRxiv : the preprint server for biology · 2026Article
- Intracellular Mechanical Stress-Mediated Autophagy Cell Death via Nanospikes for Cancer Treatment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Delamination of epithelia induced by air-liquid interfaces.Molecular biology of the cell · 2025Article
- Targeting ELOVL6 to disrupt c-MYC driven lipid metabolism in pancreatic cancer enhances chemosensitivity.Nature communications · 2025Article
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- A mathematical model of fibrinogen-mediated erythrocyte-erythrocyte adhesion.Communications biology · 2023Article
- How sticky? How tight? How hot? Imaging probes for fluid viscosity, membrane tension and temperature measurements at the cellular level.The international journal of biochemistry & cell biology · 2022Review
- Nucleus size and its effect on nucleosome stability in living cells.Biophysical journal · 2022Article
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- Interactions of platelets with circulating tumor cells contribute to cancer metastasis.Scientific reports · 2021Article
- Mechanical properties of external confinement modulate the rounding dynamics of cells.Biophysical journal · 2021Article
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- Nuclear Stiffness Decreases with Disruption of the Extracellular Matrix in Living Tissues.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
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- Engineering microscale systems for fully autonomous intracellular neural interfaces.Microsystems & nanoengineering · 2020Article
- For Whom the Bubble Grows: Physical Principles of Bubble Nucleation and Dynamics in Histotripsy Ultrasound Therapy.Ultrasound in medicine & biology · 2019Review
- Stiffness and ATP recovery of stored red blood cells in serum.Microsystems & nanoengineering · 2019Article
- Nuclear rupture at sites of high curvature compromises retention of DNA repair factors.The Journal of cell biology · 2018Article
- Importance of integrity of cell-cell junctions for the mechanics of confluent MDCK II cells.Scientific reports · 2018Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane tension underlies a range of cell physiological processes. Strong adhesion of the simple red cell is used as a simple model of a spread cell with a finite membrane tension-a state which proves useful for studies of both membrane rupture kinetics and atomic force microscopy (AFM) probing of native structure. In agreement with theories of strong adhesion, the cell takes the form of a spherical cap on a substrate densely coated with poly-L-lysine. The spreading-induced tension, sigma, in the membrane is approximately 1 mN/m, which leads to rupture over many minutes; and sigma is estimated from comparable rupture times in separate micropipette aspiration experiments. Under the sharpened tip of an AFM probe, nano-Newton impingement forces (10-30 nN) are needed to penetrate the tensed erythrocyte membrane, and these forces increase exponentially with tip velocity ( approximately nm/ms). We use the results to clarify how tapping-mode AFM imaging works at high enough tip velocities to avoid rupturing the membrane while progressively compressing it to a approximately 20-nm steric core of lipid and protein. We also demonstrate novel, reproducible AFM imaging of tension-supported membranes in physiological buffer, and we describe a stable, distended network consistent with the spectrin cytoskeleton. Additionally, slow retraction of the AFM tip from the tensed membrane yields tether-extended, multipeak sawtooth patterns of average force approximately 200 pN. In sum we show how adhesive tensioning of the red cell can be used to gain novel insights into native membrane dynamics and structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.