ArticleBiophysical journal2003
Microfabricated teflon membranes for low-noise recordings of ion channels in planar lipid bilayers.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 236 citations in OpenAlex.
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- Electrophysiological Characterization of Monoolein-Fatty Acid Bilayers.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- High-throughput single biomarker identification using droplet nanopore.Chemical science · 2024Article
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- Deep Learning-Assisted Single-Molecule Detection of Protein Post-translational Modifications with a Biological Nanopore.ACS nano · 2024Article
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- Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray.Micromachines · 2021Article
- Protein Ligand-Induced Amplification in the 1/Langmuir : the ACS journal of surfaces and colloids · 2020Article
- A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange.Micromachines · 2020Article
- Review
- The OpenPicoAmp-100k: an open-source high-performance amplifier for single channel recording in planar lipid bilayers.Pflugers Archiv : European journal of physiology · 2019Article
- Building membrane nanopores.Nature nanotechnology · 2017Review
- Enhanced Temporal Resolution with Ion Channel-Functionalized Sensors Using a Conductance-Based Measurement Protocol.Analytical chemistry · 2017Article
- Electrochemical impedance spectroscopy for black lipid membranes fused with channel protein supported on solid-state nanopore.European biophysics journal : EBJ · 2016Article
- Organellar channels and transporters.Cell calcium · 2015Review
- Shaped apertures in photoresist films enhance the lifetime and mechanical stability of suspended lipid bilayers.Biophysical journal · 2014Article
- Decreased aperture surface energy enhances electrical, mechanical, and temporal stability of suspended lipid membranes.ACS applied materials & interfaces · 2013Article
- Engineering lipid bilayer membranes for protein studies.International journal of molecular sciences · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We present a straightforward, accessible method for the fabrication of micropores with diameters from 2 to 800 micro m in films of amorphous Teflon (Teflon AF). Pores with diameters </=40 micro m made it possible to record ion fluxes through ion channels in planar bilayers with excellent signal characteristics. These pores afforded: i), stable measurements at transmembrane voltages up to 460 mV; ii), recordings at low noise levels (0.4 pA rms at 4.3 kHz bandwidth); iii), recordings at high effective bandwidth (10.7 kHz); and iv), formation of multiple planar lipid bilayers in parallel. Microfabricated pores in films of Teflon AF made it possible to examine, experimentally and theoretically, the influence of the pore diameter on the current noise in planar bilayer recordings. Reducing the pore diameter below 40 micro m mainly increased the stability of the planar bilayers, but had only a small effect on the level of the current noise. The low-noise properties of bilayer recordings on micropores in Teflon AF films were exploited to record the smallest conductance state of alamethicin (24 pS) at an unprecedentedly high bandwidth of 10.7 kHz.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.