ArticleBiophysical journal2003
Packaging of single DNA molecules by the yeast mitochondrial protein Abf2p.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
- Probing mechanical selection in diverse eukaryotic genomes through accurate prediction of 3D DNA mechanics.bioRxiv : the preprint server for biology · 2024Article
- Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae.Nucleic acids research · 2023Article
- Phase separation in genome organization across evolution.Trends in cell biology · 2021Review
- Self-assembly of multi-component mitochondrial nucleoids via phase separation.The EMBO journal · 2021Article
- Mitochondrial HMG-Box Containing Proteins: From Biochemical Properties to the Roles in Human Diseases.Biomolecules · 2020Review
- Review
- Organization of DNA in Mammalian Mitochondria.International journal of molecular sciences · 2019Review
- Prevention of mitochondrial genomic instability in yeast by the mitochondrial recombinase Mhr1.Scientific reports · 2019Article
- The role of Lon-mediated proteolysis in the dynamics of mitochondrial nucleic acid-protein complexes.Scientific reports · 2017Article
- Single-molecule studies of high-mobility group B architectural DNA bending proteins.Biophysical reviews · 2017Review
- Organization and dynamics of yeast mitochondrial nucleoids.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Review
- Yeast mitochondrial HMG proteins: DNA-binding properties of the most evolutionarily divergent component of mitochondrial nucleoids.Bioscience reports · 2015Article
- Article
- Organization of the human mitochondrial transcription initiation complex.Nucleic acids research · 2014Article
- Mechanism of homologous recombination and implications for aging-related deletions in mitochondrial DNA.Microbiology and molecular biology reviews : MMBR · 2013Review
- How environmental solution conditions determine the compaction velocity of single DNA molecules.Nucleic acids research · 2012Article
- Interactions of a replication initiator with histone H1-like proteins remodel the condensed mitochondrial genome.The Journal of biological chemistry · 2011Article
- Respiratory and TCA cycle activities affect S. cerevisiae lifespan, response to caloric restriction and mtDNA stability.Journal of bioenergetics and biomembranes · 2011Article
- Chromatin fiber dynamics under tension and torsion.International journal of molecular sciences · 2010Review
- Novel subfamily of mitochondrial HMG box-containing proteins: functional analysis of Gcf1p from Candida albicans.Microbiology (Reading, England) · 2009Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial and nuclear DNA are packaged by proteins in a very different manner. Although protein-DNA complexes called "nucleoids" have been identified as the genetic units of mitochondrial inheritance in yeast and man, little is known about their physical structure. The yeast mitochondrial protein Abf2p was shown to be sufficient to compact linear dsDNA, without the benefit of supercoiling, using optical and atomic force microscopy single molecule techniques. The packaging of DNA by Abf2p was observed to be very weak as evidenced by a fast Abf2p off-rate (k(off) = 0.014 +/- 0.001 s(-1)) and the extremely small forces (<0.6 pN) stabilizing the condensed protein-DNA complex. Atomic force microscopy images of individual complexes showed the 190-nm structures are loosely packaged relative to nuclear chromatin. This organization may leave mtDNA accessible for transcription and replication, while making it more vulnerable to damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.