ArticleBiophysical journal2003
Three-dimensional fluorescence recovery after photobleaching with the confocal scanning laser microscope.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 292 citations in OpenAlex.
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- Porcine Decellularized Diaphragm Hydrogel: A New Option for Skeletal Muscle Malformations.Biomedicines · 2021Article
- Parameter estimation in fluorescence recovery after photobleaching: quantitative analysis of protein binding reactions and diffusion.Journal of mathematical biology · 2021Article
- Enhancing nanoparticle penetration through airway mucus to improve drug delivery efficacy in the lung.Expert opinion on drug delivery · 2021Review
- DeepFRAP: Fast fluorescence recovery after photobleaching data analysis using deep neural networks.Journal of microscopy · 2021Article
- A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion.Nature communications · 2021Article
- Methods for characterizing the material properties of biomolecular condensates.Methods in enzymology · 2021Article
- Defining the Diffusion in Model Membranes Using Line Fluorescence Recovery after Photobleaching.Membranes · 2020Article
- Anomalous Diffusion Characterization by Fourier Transform-FRAP with Patterned Illumination.Biophysical journal · 2020Article
- CX3CL1 homo-oligomerization drives cell-to-cell adherence.Scientific reports · 2020Article
- Improved FRAP Measurements on Biofilms.Biophysical journal · 2020Article
- Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching.Biophysical journal · 2019Article
- Mechanical regulation of nucleocytoplasmic translocation in mesenchymal stem cells: characterization and methods for investigation.Biophysical reviews · 2019Review
- A Highly Accurate Pixel-Based FRAP Model Based on Spectral-Domain Numerical Methods.Biophysical journal · 2019Article
- Pulmonary Vascular Platform Models the Effects of Flow and Pressure on Endothelial Dysfunction inInternational journal of molecular sciences · 2018Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Confocal scanning laser microscopes (CSLMs) are equipped with the feature to photobleach user-defined regions. This makes them a handy tool to perform fluorescence recovery after photobleaching (FRAP) measurements. To allow quantification of such FRAP experiments, a three-dimensional model has been developed that describes the fluorescence recovery process for a disk-shaped geometry that is photobleached by the scanning beam of a CSLM. First the general mathematical basis is outlined describing the bleaching process for an arbitrary geometry bleached by a scanning laser beam. Next, these general expressions are applied to the bleaching by a CSLM of a disk-shaped geometry and an analytical solution is derived that describes three-dimensional fluorescence recovery in the bleached area as observed by the CSLM. The FRAP model is validated through both the Stokes-Einstein relation and the comparison of the measured diffusion coefficients with their theoretical estimates. Finally, the FRAP model is used to characterize the transport of FITC-dextrans through bulk three-dimensional biological materials: vitreous body isolated from bovine eyes, and lung sputum expectorated by cystic fibrosis patients. The decrease in the diffusion coefficient relative to its value in solution was dependent on the size of the FITC-dextrans in vitreous, whereas it was size-independent in cystic fibrosis sputum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.