ArticleProceedings of the National Academy of Sciences of the United States of America2003
Functional characterization of a portion of the Moloney murine leukemia virus gag gene by genetic footprinting.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 35 citations in OpenAlex.
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- A structure-based mechanism for tRNA and retroviral RNA remodelling during primer annealing.Nature · 2014Article
- Murine leukemia virus p12 functions include hitchhiking into the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A comparison of murine leukemia viruses that escape from human and rhesus macaque TRIM5αs.Journal of virology · 2013Article
- Assisted evolution enables HIV-1 to overcome a high TRIM5α-imposed genetic barrier to rhesus macaque tropism.PLoS pathogens · 2013Article
- Extreme genetic fragility of the HIV-1 capsid.PLoS pathogens · 2013Article
- The gammaretroviral p12 protein has multiple domains that function during the early stages of replication.Retrovirology · 2012Article
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- Evolution of endogenous retroviruses in the Suidae: evidence for different viral subpopulations in African and Eurasian host species.BMC evolutionary biology · 2011Article
- Murine leukemia viruses: objects and organisms.Advances in virology · 2011Article
- The Gag cleavage product, p12, is a functional constituent of the murine leukemia virus pre-integration complex.PLoS pathogens · 2010Article
- Protein transduction from retroviral Gag precursors.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
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- Directly monitoring individual retrovirus budding events using atomic force microscopy.Biophysical journal · 2008Article
- Mutational analysis of the N-terminal domain of Moloney murine leukemia virus capsid protein.Journal of virology · 2007Article
- Overlapping roles of the Rous sarcoma virus Gag p10 domain in nuclear export and virion core morphology.Journal of virology · 2007Article
- Evolution of feline immunodeficiency virus Gag proteins.Virus genes · 2007Article
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4 authors at 1 institution in 1 country.
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Abstract
Retroviral Gag proteins perform important functions in viral assembly, but are also involved in other steps in the viral life cycle. Conventional mutational analysis has yielded considerable information about domains essential for these functions, yet many regions of gag remain uncharacterized. We used genetic footprinting, a technique that permits the generation and simultaneous analysis of large numbers of mutations, to perform a near-saturation mutagenesis and functional analysis of 639 nucleotides in the gag region of Moloney murine leukemia virus. We report here the resulting functional map defined by eight footprints representing regions of Moloney murine leukemia virus gag, some previously uncharacterized, that are essential for replication. We found that significant portions of matrix and p12 proteins were tolerant of insertions, in contrast to the N-terminal half of capsid, which was not. We analyzed 30 mutants from our library by using conventional methods to validate the footprints. Six of these mutants were characterized in detail, identifying the precise stage at which their replication is blocked. In addition to providing the most comprehensive functional map of a retroviral gag gene, our study demonstrates the abundance of information that can be gleaned by genetic footprinting of viral sequences.
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