ArticleThe Journal of cell biology2003
Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 188 citations in OpenAlex.
- Spatiotemporal control of cortical centrin patterning by regionalized Sfi1 family scaffolding proteins in Stentor coeruleus.Current biology : CB · 2026Article
- Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin.Molecular cell · 2026Article
- Critical constituents and assembly principles of centriole biogenesis in human cells.Nature reviews. Molecular cell biology · 2026Review
- Insights into the regulation of VPS13 family bridge-like lipid transfer proteins from the structure of VPS13C.bioRxiv : the preprint server for biology · 2025Article
- Optimised Ribosome Profiling Reveals New Insights Into Translational Regulation in Synchronised Chlamydomonas reinhardtii Cultures.Plant, cell & environment · 2025Article
- Two Centrins and Their Posttranslational Modification Modulate the Cell Cycle of Giardia lamblia.MicrobiologyOpen · 2025Article
- New mutations in the core Schizosaccharomyces pombe spindle pole body scaffold Ppc89 reveal separable functions in regulating cell division.G3 (Bethesda, Md.) · 2025Article
- The core spindle pole body scaffold Ppc89 links the pericentrin orthologue Pcp1 to the fission yeast spindle pole body via an evolutionarily conserved interface.Molecular biology of the cell · 2024Article
- The relationship between intraflagellar transport and upstream protein trafficking pathways and macrocyclic lactone resistance in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2024Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2023Article
- An Sfi1-like centrin-interacting centriolar plaque protein affects nuclear microtubule homeostasis.PLoS pathogens · 2023Article
- Giant proteins in a giant cell: Molecular basis of ultrafast CaScience advances · 2023Article
- Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast.Journal of cell science · 2022Article
- Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis.The EMBO journal · 2022Article
- Conformational Plasticity of Centrin 1 fromBiomolecules · 2022Article
- Phosphorylation promotes the endonuclease-like activity of human centrin 2.RSC advances · 2022Article
- Pcp1/pericentrin controls the SPB number in fission yeast meiosis and ploidy homeostasis.The Journal of cell biology · 2022Article
- Structural and Functional Insights into the Microtubule Organizing Centers ofMicroorganisms · 2021Review
- Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition.International journal of molecular sciences · 2021Review
- Study on the interaction ofRSC advances · 2021Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centrins are calmodulin-like proteins present in microtubule-organizing centers. The Saccharomyces cerevisiae centrin, Cdc31p, was functionally tagged with a single Z domain of protein A, and used in pull-down experiments to isolate Cdc31p-binding proteins. One of these, Sfi1p, localizes to the half-bridge of the spindle pole body (SPB), where Cdc31p is also localized. Temperature-sensitive mutants in SFI1 show a defect in SPB duplication and genetic interactions with cdc31-1. Sfi1p contains multiple internal repeats that are also present in a Schizosaccharomyces pombe protein, which also localizes to the SPB, and in several human proteins, one of which localizes close to the centriole region. Cdc31p binds directly to individual Sfi1 repeats in a 1:1 ratio, so a single molecule of Sfi1p binds multiple molecules of Cdc31p. The centrosomal human protein containing Sfi1 repeats also binds centrin in the repeat region, showing that this centrin-binding motif is conserved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.