ArticleGenetics2003
A new opaque variant of maize by a single dominant RNA-interference-inducing transgene.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 230 citations in OpenAlex.
- Editing the 19 kDa alpha-zein gene family generates non-opaque2-based quality protein maize.Plant biotechnology journal · 2024Article
- Emission of floral volatiles is facilitated by cell-wall non-specific lipid transfer proteins.Nature communications · 2023Article
- Generation of multi-layered protein bodies inFrontiers in plant science · 2023Article
- Genetic and molecular understanding for the development of methionine-rich maize: a holistic approach.Frontiers in plant science · 2023Review
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Identification of a new mutant allele ofMolecular breeding : new strategies in plant improvement · 2022Article
- The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa).BMC plant biology · 2021Article
- Cuticle thickness affects dynamics of volatile emission from petunia flowers.Nature chemical biology · 2021Article
- Functional Markers for Precision Plant Breeding.International journal of molecular sciences · 2020Review
- The regulation of zein biosynthesis in maize endosperm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Intra-Kernel Reallocation of Proteins in Maize Depends on VP1-Mediated Scutellum Development and Nutrient Assimilation.The Plant cell · 2019Article
- A modular steroid-inducible gene expression system for use in rice.BMC plant biology · 2019Article
- Production of transgenic cattle expressing lysine-rich polypeptide in milk by somatic cell nuclear transfer.Transgenic research · 2019Article
- NAC-type transcription factors regulate accumulation of starch and protein in maize seeds.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Maize opaque mutants are no longer so opaque.Plant reproduction · 2018Review
- Opaque16, a high lysine and tryptophan mutant, does not influence the key physico-biochemical characteristics in maize kernel.PloS one · 2018Article
- Article
- Engineering sulfur storage in maize seed proteins without apparent yield loss.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Maize endosperm-specific transcription factors O2 and PBF network the regulation of protein and starch synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Targeted modification of storage protein content resulting in improved amino acid composition of barley grain.Transgenic research · 2016Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In maize, alpha-zeins, the main protein components of seed stores, are major determinants of nutritional imbalance when maize is used as the sole food source. Mutations like opaque-2 (o2) are used in breeding varieties with improved nutritional quality. However, o2 works in a recessive fashion by affecting the expression of a subset of 22-kD alpha-zeins, as well as additional endosperm gene functions. Thus, we sought a dominant mutation that could suppress the storage protein genes without interrupting O2 synthesis. We found that maize transformed with RNA interference (RNAi) constructs derived from a 22-kD zein gene could produce a dominant opaque phenotype. This phenotype segregates in a normal Mendelian fashion and eliminates 22-kD zeins without affecting the accumulation of other zein proteins. A system for regulated transgene expression generating antisense RNA also reduced the expression of 22-kD zein genes, but failed to give an opaque phenotype. Therefore, it appears that small interfering RNAs not only may play an important regulatory role during plant development, but also are effective genetic tools for dissecting the function of gene families. Since the dominant phenotype is also correlated with increased lysine content, the new mutant illustrates an approach for creating more nutritious crop plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.