ArticleMicrobial ecology2003
Interactions between Salmonella typhimurium and Acanthamoeba polyphaga, and observation of a new mode of intracellular growth within contractile vacuoles.
Article in Microbial ecology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- The Implication of Horizontal Gene Transfer BetweenPathogens (Basel, Switzerland) · 2026Pooled it
- The Phase-Specific Dynamics in Gene Expression of Salmonella Typhimurium During Acanthamoeba castellanii Infection.Current microbiology · 2025Article
- Acanthamoeba spp.: Neglected Protists in Veterinary Medicine.Acta parasitologica · 2025Review
- Survival and genomic stability ofFrontiers in cellular and infection microbiology · 2025Article
- Biofilm formation of Salmonella enterica serovar Enteritidis cocultured with Acanthamoeba castellanii responds to nutrient availability.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- War of the microbial world: Acanthamoeba spp. interactions with microorganisms.Folia microbiologica · 2021Review
- Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence forInternational journal of molecular sciences · 2021Article
- Type 1 Fimbriae and Motility Play a Pivotal Role During Interactions of Salmonella typhimurium with Acanthamoeba castellanii (T4 Genotype).Current microbiology · 2020Article
- The cellulose synthase BcsA plays a role in interactions of Salmonella typhimurium with Acanthamoeba castellanii genotype T4.Parasitology research · 2018Article
- Presence and Persistence ofFrontiers in public health · 2018Review
- Free-Living Amoebae as Hosts for and Vectors of Intracellular Microorganisms with Public Health Significance.Viruses · 2017Review
- Packaging of Campylobacter jejuni into Multilamellar Bodies by the Ciliate Tetrahymena pyriformis.Applied and environmental microbiology · 2016Article
- An intracellular replication niche for Vibrio cholerae in the amoeba Acanthamoeba castellanii.The ISME journal · 2016Article
- Relevant Genes Linked to Virulence Are Required for Salmonella Typhimurium to Survive Intracellularly in the Social Amoeba Dictyostelium discoideum.Frontiers in microbiology · 2016Article
- Draft Genome Sequence of Shewanella sp. Strain CP20.Genome announcements · 2015Article
- Review
- Behavior of Yersinia enterocolitica in the presence of the bacterivorous Acanthamoeba castellanii.Applied and environmental microbiology · 2013Article
- Evaluation of the potential antimicrobial resistance transfer from a multi-drug resistant Escherichia coli to Salmonella in dairy calves.Current microbiology · 2013Article
- Increased persistence of Salmonella enterica serovar Typhi in the presence of Acanthamoeba castellanii.Applied and environmental microbiology · 2011Article
- Detection of Vibrio cholerae and Acanthamoeba species from same natural water samples collected from different cholera endemic areas in Sudan.BMC research notes · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acanthamoeba polyphaga feeding on Salmonella typhimurium in a simple model biofilm were observed by light microscopy and a detailed record of interactions kept by digital image capture and image analysis. A strain of S. typhimurium SL1344 carrying a fis: gfp reporter construct (pPDT105) was used to assess intracellular growth in A. polyphaga on non-nutrient agar (NNA) plates. Invasion of the contractile vacuole (CV) was observed at a frequency of 1:100-1000 acanthamoebae at 35 degrees C. The salmonellae contained in CVs illustrated significant up-regulation of fis relative to extracellular bacteria, indicating that they were in the early stages of logarithmic growth, and reached numbers of 100-200 cells per vacuole after 4 days. This is the first report of this mode of intracellular growth. Up-regulation of fis was also observed in a proportion of S. typhimurium cells contained within food vacuoles. Filamentation of S. typhimurium and E. coli cells was frequently observed in coculture with A. polyphaga on NNA plates, with bacterial cells reaching lengths of up to 500 microm after 10 days' incubation at 35 degrees C. A. polyphaga was also seen to mediate bacterial translocation over the agar surface; egested salmonellae subsequently formed microcolonies along amoebal tracks. This illustrated intracellular survival of a fraction of the S. typhimurium population. These phenomena suggest that protozoa such as A. polyhaga may play an important role in the ecology of S. typhimurium in soil and aquatic environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.