ArticleGene therapy2003
Development of a novel gene delivery scaffold utilizing colloidal gold-polyethylenimine conjugates for DNA condensation.
Article in Gene therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Polyethyleneimine/polyethylene glycol-conjugated gold nanoparticles as nanoscale positive/negative controls in nanotoxicology: testing in frog embryo teratogenesis assay-Nanotoxicology · 2023Article
- Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro.International journal of nanomedicine · 2021Article
- Parallel multi-parameter study of PEI-functionalized gold nanoparticle synthesis for bio-medical applications: part 1-a critical assessment of methodology, properties, and stability.Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology · 2019Article
- Scaffold-mediated delivery for non-viral mRNA vaccines.Gene therapy · 2018Article
- Histone-Mimetic Gold Nanoparticles as Versatile Scaffolds for Gene Transfer and Chromatin Analysis.Bioconjugate chemistry · 2018Article
- An efficient, non-viral dendritic vector for gene delivery in tissue engineering.Gene therapy · 2017Article
- Dendronized gold nanoparticles for siRNA delivery.Small (Weinheim an der Bergstrasse, Germany) · 2012Article
- Transgene delivery using poly(amino ether)-gold nanorod assemblies.Biotechnology and bioengineering · 2012Article
- Rheological and dielectric properties of different gold nanoparticle sizes.Lipids in health and disease · 2011Article
- Biocompatibility of Fe₃O₄@Au composite magnetic nanoparticles in vitro and in vivo.International journal of nanomedicine · 2011Article
- Spatiotemporal temperature distribution and cancer cell death in response to extracellular hyperthermia induced by gold nanorods.ACS nano · 2010Article
- Simultaneous enhancement of photothermal stability and gene delivery efficacy of gold nanorods using polyelectrolytes.ACS nano · 2009Article
- Polymeric Materials for Gene Delivery and DNA Vaccination.Advanced materials (Deerfield Beach, Fla.) · 2009Article
- Degradable polymers for gene delivery.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2009Article
- In vivo expression of myosin essential light chain using plasmid expression vectors in regenerating frog skeletal muscle.Gene therapy · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a novel gene delivery scaffold based on DNA plasmid condensation with colloidal gold/polyethylenimine conjugates. This scaffold system was designed to enable systematic study of the relationships between DNA complex physical properties and transfection efficiency. Using an enhanced green fluorescent protein-coding reporter plasmid and a Chinese hamster ovary cell line, we have measured the transfection efficiencies of our complexes using flow cytometry and their cytotoxicities using the trypan blue assay. We have also assayed complex particle morphologies using atomic force microscopy, photon correlation spectroscopy, and a novel plasmon absorbance peak position analysis. We achieved comparable rates of transfection relative to the commonly used polycationic condensation agents calcium phosphate and LipofectAMINE, with comparably low cytotoxicities. In addition, by manipulating colloidal gold concentration, we could partially decouple complex physical properties including charge ratio, size, DNA loading, and polyethylenimine concentration. Our morphological analyses showed that complexes with a diameter of a few hundred nanometers and a charge ratio of approximately 8 perform best in our transfection efficiency assays. The use of colloidal gold as a component in our delivery system provides a versatile system for manipulating complex properties and morphology as well as a convenient scaffold for planned ligand conjugation studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.