ArticleNucleic acids research2003
DNA display for in vitro selection of diverse peptide libraries.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 98 citations in OpenAlex.
- Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- "Click handle"-modified 2'-deoxy-2'-fluoroarabino nucleic acid as a synthetic genetic polymer capable of post-polymerization functionalization.Chemical science · 2022Article
- Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins.Nucleic acids research · 2020Article
- Evolving a Peptide: Library Platforms and Diversification Strategies.International journal of molecular sciences · 2019Review
- A Genetically Encoded, Phage-Displayed Cyclic-Peptide Library.Angewandte Chemie (International ed. in English) · 2019Review
- High-throughput screening of biomolecules using cell-free gene expression systems.Synthetic biology (Oxford, England) · 2018Review
- Peptide aptamers: development and applications.Current topics in medicinal chemistry · 2015Review
- Technologies for the synthesis of mRNA-encoding libraries and discovery of bioactive natural product-inspired non-traditional macrocyclic peptides.Molecules (Basel, Switzerland) · 2013Review
- Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution.Nucleic acids research · 2013Article
- A highly scalable peptide-based assay system for proteomics.PloS one · 2012Article
- Detection of biomarkers using recombinant antibodies coupled to nanostructured platforms.Nano reviews · 2012Article
- DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells.PloS one · 2012Article
- Uniform amplification of phage with different growth characteristics in individual compartments consisting of monodisperse droplets.Angewandte Chemie (International ed. in English) · 2010Article
- Bacterial display enables efficient and quantitative peptide affinity maturation.Protein engineering, design & selection : PEDS · 2010Article
- Bicistronic DNA display for in vitro selection of Fab fragments.Nucleic acids research · 2009Article
- An efficient method to assemble linear DNA templates for in vitro screening and selection systems.Nucleic acids research · 2009Article
- Directed evolution of streptavidin variants using in vitro compartmentalization.Chemistry & biology · 2008Article
- Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides.Protein engineering, design & selection : PEDS · 2008Article
- The detection limit of a Gd3+-based T1 agent is substantially reduced when targeted to a protein microdomain.Magnetic resonance imaging · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the use of a DNA display system for in vitro selection of peptide ligands from a large library of peptides displayed on their encoding DNAs. The method permits completely in vitro construction of a DNA-tagged peptide library by using a wheat germ in vitro transcription/translation system compartmentalized in water-in-oil emulsions. Starting with a library of 10(9)-10(10) random decapeptides, 21 different peptide ligands were isolated for monoclonal antibody anti-FLAG M2. DNA display selected more diverse peptides with a DYKXXD consensus motif than previously reported phage display systems. Binding and recovery rates of three peptides were significantly higher than those of the original FLAG peptide, implying that these peptides would be superior to the FLAG peptide for purification of tagged proteins. The simplicity of DNA display enables two selection rounds per day to be conducted. Further, DNA display can overcome the limitations of previous display technologies by avoiding the use of bacterial cells and RNA tags. Thus, DNA display is expected to be useful for rapid screening of a wide variety of peptide ligands for corresponding receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.