ArticleNucleic acids research2003
A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 64 citations in OpenAlex.
- Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays.Scientific reports · 2021Article
- Genetic diversity in European Pisum germplasm collections.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Review
- iPBS: a universal method for DNA fingerprinting and retrotransposon isolation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- The genetic diversity and evolution of field pea (Pisum) studied by high throughput retrotransposon based insertion polymorphism (RBIP) marker analysis.BMC evolutionary biology · 2010Article
- Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Rice molecular breeding laboratories in the genomics era: Current status and future considerations.International journal of plant genomics · 2008Article
- Gene-based sequence diversity analysis of field pea (Pisum).Genetics · 2007Article
- Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification.Journal of applied genetics · 2006Article
- Validation of the high-throughput marker technology DArT using the model plant Arabidopsis thaliana.Molecular genetics and genomics : MGG · 2005Article
- Comparative analyses of genetic diversities within tomato and pepper collections detected by retrotransposon-based SSAP, AFLP and SSR.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Studying plant genome variation using molecular markers.Russian journal of genetics · 2005Article
- Novel hairpin-shaped primer assay to study the association of the -44 single-nucleotide polymorphism of the DEFB1 gene with early-onset periodontal disease.Clinical and diagnostic laboratory immunology · 2004Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotin- terminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allele- specific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.