ArticleNucleic acids research2003
Characterization of the RNA motif responsible for the specific interaction of potato spindle tuber viroid RNA (PSTVd) and the tomato protein Virp1.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 103 citations in OpenAlex.
- Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication.Plants (Basel, Switzerland) · 2024Review
- Review
- RNA three-dimensional structure drives the sequence organization of potato spindle tuber viroid quasispecies.PLoS pathogens · 2024Article
- Viroid Replication, Movement, and the Host Factors Involved.Microorganisms · 2024Review
- Cellular roadmaps of viroid infection.Trends in microbiology · 2023Review
- Article
- Viroids: Non-Coding Circular RNAs Able to Autonomously Replicate and Infect Higher Plants.Biology · 2023Review
- Circular RNAs: biology and clinical significance of breast cancer.RNA biology · 2023Review
- A nuclear import pathway exploited by pathogenic noncoding RNAs.The Plant cell · 2022Article
- Emerging value of the viroid model in molecular biology and beyond.Virus research · 2022Review
- Conserved Motifs and Domains in Members ofCells · 2022Article
- Studies on Viroid Shed Light on the Role of RNA Three-Dimensional Structural Motifs in RNA Trafficking in Plants.Frontiers in plant science · 2022Review
- Predicting the Structure of a Viroid : Structure, Structure Distribution, Consensus Structure, and Structure Drawing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Symptom Severity, Infection Progression and Plant Responses in Solanum Plants Caused by Three Pospiviroids Vary with the Inoculation Procedure.International journal of molecular sciences · 2021Article
- Review
- Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).Biomolecules · 2021Article
- RNA Motifs and Modification Involve in RNA Long-Distance Transport in Plants.Frontiers in cell and developmental biology · 2021Review
- Whole-Genome Deep Sequencing Reveals Host-DrivenInternational journal of molecular sciences · 2020Article
- A three-dimensional RNA motif mediates directional trafficking of Potato spindle tuber viroid from epidermal to palisade mesophyll cells in Nicotiana benthamiana.PLoS pathogens · 2019Article
- Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viroids are small non-coding parasitic RNAs that are able to infect their host plants systemically. This circular naked RNA makes use of host proteins to accomplish its proliferation. Here we analyze the specific binding of the tomato protein Virp1 to the terminal right domain of potato spindle tuber viroid RNA (PSTVd). We find that two asymmetric internal loops within the PSTVd (+) RNA, each composed of the sequence elements 5'-ACAGG and CUCUUCC-5', are responsible for the specific RNA-protein interaction. In view of the nucleotide composition we call this structural element an 'RY motif'. The RY motif located close to the terminal right hairpin loop of the PSTVd secondary structure has an approximately 5-fold stronger binding affinity than the more centrally located RY motif. Simultaneous sequence alterations in both RY motifs abolished the specific binding to Virp1. Mutations in any of the two RY motifs resulted in non-infectious viroid RNA, with the exception of one case, where reversion to sequence wild type took place. In contrast, the simultaneous exchange of two nucleotides within the terminal right hairpin loop of PSTVd had only moderate influence on the binding to Virp1. This variant was infectious and sequence changes were maintained in the progeny. The relevance of the phylogenetic conservation of the RY motif, and sequence elements therein, amongst various genera of the family Pospiviroidae is discussed.
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