ArticleThe Journal of physiology2003
Glutamate-mediated cytosolic calcium oscillations regulate a pulsatile prostaglandin release from cultured rat astrocytes.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 187 citations in OpenAlex.
- Astrocytes Coordinate Neurovascular Coupling During Prolonged Sensory Demand via AQP4/TRPA1/BEST1/D-Serine Signaling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Active Astrocyte: Calcium Dynamics, Circuit Modulation, and Targets for Intervention.Neurochemical research · 2025Review
- High-Fat-Diet-Induced Metabolic Disorders: An Original Cause for Neurovascular Uncoupling Through the Imbalance of Glutamatergic Pathways.Biomedicines · 2025Article
- Unveiling the Interplay: Neurovascular Coupling, Astrocytes and G Protein-Coupled Receptors in Alzheimer's Disease.ACS pharmacology & translational science · 2025Review
- Article
- Astrocytic GPCR-Induced CaInternational journal of molecular sciences · 2023Article
- Two decades of astrocytes in neurovascular coupling.Frontiers in network physiology · 2023Review
- Review
- Metabolic Contribution and Cerebral Blood Flow Regulation by Astrocytes in the Neurovascular Unit.Cells · 2022Review
- In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways.Scientific reports · 2021Article
- Impaired Hippocampal Neurovascular Coupling in a Mouse Model of Alzheimer's Disease.Frontiers in physiology · 2021Article
- Retinal Structural and Microvascular Alterations in Different Acute Ischemic Stroke Subtypes.Journal of ophthalmology · 2020Article
- Simulation of calcium signaling in fine astrocytic processes: Effect of spatial properties on spontaneous activity.PLoS computational biology · 2019Article
- Augmented astrocyte microdomain CaGlia · 2019Article
- Impaired lipid metabolism markers to assess the risk of neuroinflammation in autism spectrum disorder.Metabolic brain disease · 2018Article
- Disrupted Neuroglial Metabolic Coupling after Peripheral Surgery.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Prostaglandin EFrontiers in pharmacology · 2017Article
- Beyond neurovascular coupling, role of astrocytes in the regulation of vascular tone.Neuroscience · 2016Review
- 3,4-methylenedioxymethamphetamine increases excitability in the dentate gyrus: role of 5HT2A receptor-induced PGE2 signaling.Journal of neurochemistry · 2016Article
- The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i.BioMed research international · 2016Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The synaptic release of glutamate evokes in astrocytes periodic increases in [Ca2+]i, due to the activation of metabotropic glutamate receptors (mGluRs). The frequency of these [Ca2+]i oscillations is controlled by the level of neuronal activity, indicating that they represent a specific, frequency-coded signalling system of neuron-to-astrocyte communication. We recently found that neuronal activity-dependent [Ca2+]i oscillations in astrocytes are the main signal that regulates the coupling between neuronal activity and blood flow, the so-called functional hyperaemia. Prostaglandins play a major role in this fundamental phenomenon in brain function, but little is known about a possible link between [Ca2+]i oscillations and prostaglandin release from astrocytes. To investigate whether [Ca2+]i oscillations regulate the release of vasoactive prostaglandins, such as the potent vasodilator prostaglandin E2 (PGE2), from astrocytes, we plated wild-type human embryonic kidney (HEK)293 cells, which respond constitutively to PGE2 with [Ca2+]i elevations, onto cultured astrocytes, and used them as biosensors of prostaglandin release. After loading the astrocyte-HEK cell co-cultures with the calcium indicator Indo-1, confocal microscopy revealed that mGluR-mediated [Ca2+]i oscillations triggered spatially and temporally coordinated [Ca2+]i increases in the sensor cells. This response was absent in a clone of HEK cells that are unresponsive to PGE2, and recovered after transfection with the InsP3-linked prostanoid receptor EP1. We conclude that [Ca2+]i oscillations in astrocytes regulate prostaglandin releases that retain the oscillatory behaviour of the [Ca2+]i changes. This finely tuned release of PGE2 from astrocytes provides a coherent mechanistic background for the role of these glial cells in functional hyperaemia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.