ArticleThe Journal of physiology2003
Effects of tropomyosin internal deletion Delta23Tm on isometric tension and the cross-bridge kinetics in bovine myocardium.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 26 citations in OpenAlex.
- Effects of cooling on pig heart excitation and contraction.Frontiers in cardiovascular medicine · 2026Article
- Biomechanical evaluation of flash-frozen and cryo-sectioned papillary muscle samples by using sinusoidal analysis: cross-bridge kinetics and the effect of partial CaJournal of muscle research and cell motility · 2024Article
- Development and prevention of ischemic contracture ("stone heart") in the pig heart.Frontiers in cardiovascular medicine · 2023Article
- The effect of gender and obesity in modulating cross-bridge function in cardiac muscle fibers.Journal of muscle research and cell motility · 2022Article
- Troponin Revealed: Uncovering the Structure of the Thin Filament On-Off Switch in Striated Muscle.Biophysical journal · 2021Review
- Functional significance of HCM mutants of tropomyosin, V95A and D175N, studied withBiophysics and physicobiology · 2019Article
- A study of tropomyosin's role in cardiac function and disease using thin-filament reconstituted myocardium.Journal of muscle research and cell motility · 2013Review
- Analysis of the molecular pathogenesis of cardiomyopathy-causing cTnT mutants I79N, ΔE96, and ΔK210.Biophysical journal · 2013Article
- A periodic pattern of evolutionarily conserved basic and acidic residues constitutes the binding interface of actin-tropomyosin.The Journal of biological chemistry · 2013Article
- Nitroxyl-mediated disulfide bond formation between cardiac myofilament cysteines enhances contractile function.Circulation research · 2012Article
- Article
- The role of tropomyosin domains in cooperative activation of the actin-myosin interaction.Journal of molecular biology · 2011Article
- Enhanced active cross-bridges during diastole: molecular pathogenesis of tropomyosin's HCM mutations.Biophysical journal · 2011Article
- The role of tropomyosin isoforms and phosphorylation in force generation in thin-filament reconstituted bovine cardiac muscle fibres.Journal of muscle research and cell motility · 2010Article
- Mechanical and kinetic effects of shortened tropomyosin reconstituted into myofibrils.Pflugers Archiv : European journal of physiology · 2009Article
- Tropomyosin period 3 is essential for enhancement of isometric tension in thin filament-reconstituted bovine myocardium.Journal of biophysics (Hindawi Publishing Corporation : Online) · 2009Article
- Temperature-dependence of isometric tension and cross-bridge kinetics of cardiac muscle fibers reconstituted with a tropomyosin internal deletion mutant.Biophysical journal · 2006Article
- Temperature change does not affect force between regulated actin filaments and heavy meromyosin in single-molecule experiments.The Journal of physiology · 2006Article
- Use of thin filament reconstituted muscle fibres to probe the mechanism of force generation.Journal of muscle research and cell motility · 2006Review
- Elementary steps of the cross-bridge cycle in fast-twitch fiber types from rabbit skeletal muscles.Biophysical journal · 2005Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Tropomyosin (Tm) spans seven actin monomers and contains seven quasi-repeating, loosely similar regions, 1-7. Deletion of regions 2-3 decreases the in vitro sliding speed of synthetic filaments of actin-Tm-Troponin (Tn), and weakens Tm binding to the actin-myosin subfragment 1 (S1) complex (acto-S1). The thin filament was selectively removed from bovine myocardium by gelsolin, and the actin filament was reconstituted, followed by further reconstitution with Tm and Tn. In this reconstitution, full-length Tm (control) was compared with Tm internal deletion mutant Delta23Tm, which lacks residues 47-123 (regions 2-3). The effects of phosphate, MgATP, MgADP and Ca2+ were studied in Tm-reconstituted myocardium and Delta23Tm-reconstituted myocardium at pH 7.00 and 25 degrees C. In Delta23Tm, both isometric tension and stiffness were about 40 % of the control. The Hill factor with Delta23Tm, deduced from the pCa-tension plot, was 1.4 times that of the control, but the Ca2+ sensitivity was the same. Sinusoidal analysis indicated that the cross-bridge number in force-generating states was not decreased with Delta23Tm. We conclude that the thin filament cooperativity is increased with Delta23Tm, presumably because of the increased density of the Ca2+-binding sites. We further conclude that tension per cross-bridge is 40 % of control and stiffness per cross-bridge is 40 % of control in Delta23Tm. These results are consistent with the idea that Tm modifies the actin-myosin interface so as to increase the stereospecific interaction between moieties of actin and myosin. In Delta23Tm, the interface may not have a perfect stereospecific match so that the tension- and stiffness-generating capacity is greatly diminished.
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