ArticleInfection and immunity2003
Construction and evaluation of a safe, live, oral Vibrio cholerae vaccine candidate, IEM108.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Role of the TPR family protein VPA1365 in regulating type III secretion system 2 and virulence inApplied and environmental microbiology · 2025Article
- Challenges and Considerations in Selecting Animal Models for Evaluating a Live, Cold-Chain-Free, Dual-Use Vaccine (MyChol) for Diarrhoeal Diseases: A Comprehensive Review.The Malaysian journal of medical sciences : MJMS · 2024Review
- Helicobacter pylori.Current topics in microbiology and immunology · 2024Review
- Identification of determinants for entering into a viable but nonculturable state in Vibrio alginolyticus by Tn-seq.Applied microbiology and biotechnology · 2023Article
- Transcriptome Analysis Reveals the Effect of Low NaCl Concentration on Osmotic Stress and Type III Secretion System inInternational journal of molecular sciences · 2023Article
- Review
- A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility inPathogens (Basel, Switzerland) · 2022Article
- Vibrio parahaemolyticus CadC regulates acid tolerance response to enhance bacterial motility and cytotoxicity.Journal of fish diseases · 2021Article
- An Update on Cholera Immunity and Current and Future Cholera Vaccines.Tropical medicine and infectious disease · 2021Article
- Article
- Deciphering biophysical signatures for microbiological applications.Lasers in medical science · 2020Article
- Immunogenicity and protective efficacy of a live, oral cholera vaccine formulation stored outside-the-cold-chain for 140 days.BMC immunology · 2020Article
- Alternative Sigma Factor RpoX Is a Part of the RpoE Regulon and Plays Distinct Roles in Stress Responses, Motility, Biofilm Formation, and Hemolytic Activities in the Marine Pathogen Vibrio alginolyticus.Applied and environmental microbiology · 2019Article
- Identification of the Regulon of AphB and Its Essential Roles in LuxR and Exotoxin Asp Expression in the Pathogen Vibrio alginolyticus.Journal of bacteriology · 2017Article
- Article
- The Hybrid Pre-CTXΦ-RS1 Prophage Genome and Its Regulatory Function in Environmental Vibrio cholerae O1 Strains.Applied and environmental microbiology · 2015Article
- CTXφ Replication Depends on the Histone-Like HU Protein and the UvrD Helicase.PLoS genetics · 2015Article
- Vaccines for viral and bacterial pathogens causing acute gastroenteritis: Part I: Overview, vaccines for enteric viruses and Vibrio cholerae.Human vaccines & immunotherapeutics · 2015Review
- Genomic science in understanding cholera outbreaks and evolution of Vibrio cholerae as a human pathogen.Current topics in microbiology and immunology · 2014Review
- Vaccines against human diarrheal pathogens: current status and perspectives.Human vaccines & immunotherapeutics · 2014Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
IEM101, a Vibrio cholerae O1 El Tor Ogawa strain naturally deficient in CTXPhi, was previously selected as a live cholera vaccine candidate. To make a better and safer vaccine that can induce protective immunity against both the bacteria and cholera toxin (CT), a new vaccine candidate, IEM108, was constructed by introducing a ctxB gene and an El Tor-derived rstR gene into IEM101. The ctxB gene codes for the protective antigen CTB subunit, and the rstR gene mediates phage immunity. The stable expression of the two genes was managed by a chromosome-plasmid lethal balanced system based on the housekeeping gene thyA. Immunization studies indicate that IEM108 generates good immune responses against both the bacteria and CT. After a single-dose intraintestinal vaccination with 10(9) CFU of IEM108, both anti-CTB immunoglobulin G and vibriocidal antibodies were detected in the immunized-rabbit sera. However, only vibriocidal antibodies are detected in rabbits immunized with IEM101. In addition, IEM108 but not IEM101 conferred full protection against the challenges of four wild-type toxigenic strains of V. cholerae O1 and 4 micro g of CT protein in a rabbit model. By introducing the rstR gene, the frequency of conjugative transfer of a recombinant El Tor-derived RS2 suicidal plasmid to IEM108 was decreased 100-fold compared to that for IEM101. This indicated that the El Tor-derived rstR cloned in IEM108 was fully functional and could effectively inhibit the El Tor-derived CTXPhi from infecting IEM108. Our results demonstrate that IEM108 is an efficient and safe live oral cholera vaccine candidate that induces antibacterial and antitoxic immunity and CTXPhi phage immunity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.