ArticleMolecular and cellular biology1992
Isolation and structural analysis of a 1.2-megabase N-myc amplicon from a human neuroblastoma.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 55 citations in OpenAlex.
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- Discoveries of Extrachromosomal Circles of DNA in Normal and Tumor Cells.Trends in genetics : TIG · 2018Review
- A chemical screen identifies the chemotherapeutic drug topotecan as a specific inhibitor of the B-MYB/MYCN axis in neuroblastoma.Oncotarget · 2012Article
- Overexpression of SERTAD3, a putative oncogene located within the 19q13 amplicon, induces E2F activity and promotes tumor growth.Oncogene · 2007Article
- Molecular structure of double-minute chromosomes bearing amplified copies of the epidermal growth factor receptor gene in gliomas.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Gene amplification in PNETs/medulloblastomas: mapping of a novel amplified gene within the MYCN amplicon.Journal of medical genetics · 2000Article
- MYCN gene amplification. Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors.The American journal of pathology · 1999Article
- The evolution of small DNA viruses of eukaryotes: past and present considerations.Virus genes · 1995Review
- Rapid detection, cloning and molecular cytogenetic characterisation of sequences from an MRP-encoding amplicon by chromosome microdissection.British journal of cancer · 1994Article
- MYCN is retained in single copy at chromosome 2 band p23-24 during amplification in human neuroblastoma cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Megabase-scale analysis of the origin of N-myc amplicons in human neuroblastomas.Nucleic acids research · 1994Article
- CpG island mapping of a mouse double-minute chromosome.Molecular and cellular biology · 1993Article
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7 authors at 1 institution in 1 country.
Funding
Abstract
Oncogene amplification is observed frequently in human cancers, but little is known about the mechanism of gene amplification or the structure of amplified DNA in tumor cells. We have studied the N-myc amplified domain from a representative neuroblastoma cell line, SMS-KAN, and compared the map of the amplicon in this cell line with that seen in normal DNA. The SMS-KAN cell line DNA was cloned into yeast artificial chromosomes (YACs), and clones were identified by screening the YAC library with amplified DNA probes that were obtained previously (B. Zehnbauer, D. Small, G. M. Brodeur, R. Seeger, and B. Vogelstein, Mol. Cell. Biol. 8:522-530, 1988). In addition, YAC clones corresponding to the normal N-myc locus on chromosome 2 were obtained by screening two normal human YAC libraries with these probes, and the restriction maps of the two sets of overlapping YACs were compared. Our results suggest that the amplified domain in this cell line is a approximately 1.2-Mb circular molecule with a head-to-tail configuration, and the physical map of the normal N-myc locus generally is conserved in the amplicon. These results provide a physical map of the amplified domain of a neuroblastoma cell line that has de novo amplification of an oncogene. The head-to-tail organization, the general conservation of the normal physical map in the amplicon, and the extrachromosomal location of the amplified DNA are most consistent with the episome formation-plus-segregation mechanism of gene amplification in these tumors.
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