ArticleThe Journal of cell biology1992
Okadaic acid induces interphase to mitotic-like microtubule dynamic instability by inactivating rescue.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Emergent microtubule properties in a model of filament turnover and nucleation.Journal of theoretical biology · 2026Article
- Minimal Mechanisms of Microtubule Length Regulation in Living Cells.Bulletin of mathematical biology · 2024Article
- Predicted Effects of Severing Enzymes on the Length Distribution and Total Mass of Microtubules.Biophysical journal · 2019Article
- LB100, a small molecule inhibitor of PP2A with potent chemo- and radio-sensitizing potential.Cancer biology & therapy · 2015Review
- 3-(Methoxycarbonylmethylene)isobenzofuran-1-imines as a new class of potential herbicides.Molecules (Basel, Switzerland) · 2014Article
- In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics.Developmental cell · 2014Article
- Article
- Kif18A uses a microtubule binding site in the tail for plus-end localization and spindle length regulation.Current biology : CB · 2011Article
- Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos.Development (Cambridge, England) · 2010Article
- MCAK and paclitaxel have differential effects on spindle microtubule organization and dynamics.Molecular biology of the cell · 2009Article
- Article
- Protein tyrosine and serine-threonine phosphatases in the sea urchin, Strongylocentrotus purpuratus: identification and potential functions.Developmental biology · 2006Article
- Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification.The Journal of cell biology · 2003Article
- Unwinding the loop of Bcl-2 phosphorylation.Leukemia · 2001Review
- Dissociation of the tubulin-sequestering and microtubule catastrophe-promoting activities of oncoprotein 18/stathmin.Molecular biology of the cell · 1999Article
- Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling.The Journal of cell biology · 1997Article
- Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.The Journal of cell biology · 1997Article
- A metastable intermediate state of microtubule dynamic instability that differs significantly between plus and minus ends.The Journal of cell biology · 1997Article
- Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro.Molecular biology of the cell · 1997Article
- Evidence that a single monolayer tubulin-GTP cap is both necessary and sufficient to stabilize microtubules.Molecular biology of the cell · 1996Article
Corrections and comments
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3 authors.
Funding
Abstract
We used high-resolution video microscopy to visualize microtubule dynamic instability in extracts of interphase sea urchin eggs and to analyze the changes that occur upon addition of 0.8-2.5 microM okadaic acid, an inhibitor of phosphatase 1 and 2A (PP1, PP2a) (Bialojan, D., and A. Takai. 1988. Biochem. J. 256:283-290). Microtubule plus-ends in these extracts oscillated between the elongation and shortening phases of dynamic instability at frequencies typical for interphase cells. Switching from elongation to shortening (catastrophe) was frequent, but microtubules persisted and grew long because of frequent switching back to elongation (rescue). Addition of okadaic acid to the extract induced rapid (< 5 min) conversion to short, dynamic microtubules typical of mitosis. The frequency of catastrophe doubled and the velocities of elongation and shortening increased slightly; however, the major change was an elimination of rescue. Thus, modulation of the rescue frequency by phosphorylation-dependent mechanisms may be a major regulatory pathway for selectively controlling microtubule dynamics without dramatically changing velocities of microtubule elongation and shortening.
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