ArticleThe Journal of cell biology1992
Thymosin beta 4 sequesters the majority of G-actin in resting human polymorphonuclear leukocytes.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 158 citations in OpenAlex.
- Thymosin β4 promotes zebrafish Mauthner axon regeneration by facilitating actin polymerization through binding to G-actin.BMC biology · 2024Article
- The role of the cytoskeleton in fibrotic diseases.Frontiers in cell and developmental biology · 2024Review
- Thymosin β4 Is an Endogenous Iron Chelator and Molecular Switcher of Ferroptosis.International journal of molecular sciences · 2022Article
- Article
- Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm Transcriptome by RNA-Seq.Frontiers in veterinary science · 2021Article
- Thymosin β4 is essential for adherens junction stability and epidermal planar cell polarity.Development (Cambridge, England) · 2020Article
- Moonlighting Proteins in the Fuzzy Logic of Cellular Metabolism.Molecules (Basel, Switzerland) · 2020Review
- No effect of thymosin beta-4 on the expression of the transcription factor Islet-1 in the adult murine heart.Pharmacology research & perspectives · 2018Article
- Protein moonlighting: what is it, and why is it important?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Capping protein regulators fine-tune actin assembly dynamics.Nature reviews. Molecular cell biology · 2014Review
- Novel bacterial ADP-ribosylating toxins: structure and function.Nature reviews. Microbiology · 2014Review
- In vivo growth suppression of CT-26 mouse colorectal cancer cells by adenovirus-expressed small hairpin RNA specifically targeting thymosin beta-4 mRNA.Cancer gene therapy · 2014Article
- Interaction of profilin with the barbed end of actin filaments.Biochemistry · 2013Article
- Expression patterns of Thymosin β4 and cancer stem cell marker CD133 in ovarian cancers.Pathology oncology research : POR · 2013Article
- Article
- Conformational dynamics of actin: effectors and implications for biological function.Cytoskeleton (Hoboken, N.J.) · 2010Review
- Thymosin beta4: a key factor for protective effects of eEPCs in acute and chronic ischemia.Annals of the New York Academy of Sciences · 2010Article
- Thymosin beta 4: A novel corneal wound healing and anti-inflammatory agent.Clinical ophthalmology (Auckland, N.Z.) · 2007Article
- Roles of thymosins in cancers and other organ systems.World journal of surgery · 2005Review
- Overexpression of the thymosin beta-4 gene is associated with malignant progression of SW480 colon cancer cells.Oncogene · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Thymosin beta 4 (T beta 4), a 5-kD peptide which binds G-actin and inhibits its polymerization (Safer, D., M. Elzinga, and V. T. Nachmias. 1991. J. Biol. Chem. 266:4029-4032), appears to be the major G-actin sequestering protein in human PMNs. In support of a previous study by Hannappel, E., and M. Van Kampen (1987. J. Chromatography. 397:279-285), we find that T beta 4 is an abundant peptide in these cells. By reverse phase HPLC of perchloric acid supernatants, human PMNs contain approximately 169 fg/cell +/- 90 fg/cell (SD), corresponding to a cytoplasmic concentration of approximately 149 +/- 80.5 microM. On non-denaturing polyacrylamide gels, a large fraction of G-actin in supernatants prepared from resting PMNs has a mobility similar to the G-actin/T beta 4 complex. Chemoattractant stimulation of PMNs results in a decrease in this G-actin/T beta 4 complex. To determine whether chemoattractant induced actin polymerization results from an inactivation of T beta 4, the G-actin sequestering activity of supernatants prepared from resting and chemoattractant stimulated cells was measured by comparing the rates of pyrenyl-actin polymerization from filament pointed ends. Pyrenyl actin polymerization was inhibited to a greater extent in supernatants from stimulated cells and these results are qualitatively consistent with T beta 4 being released as G-actin polymerizes, with no chemoattractant-induced change in its affinity for G-actin. The kinetics of bovine spleen T beta 4 binding to muscle pyrenyl G-actin are sufficiently rapid to accommodate the rapid changes in actin polymerization and depolymerization observed in vivo in response to chemoattractant addition and removal.
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