ArticleProceedings of the National Academy of Sciences of the United States of America1992
Gene family for an elicitor-induced sesquiterpene cyclase in tobacco.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 249 citations in OpenAlex.
- Mechanistic Characterisation of Bacterial Terpene Synthases from Chitinophagaceae Producing Marine-Type Diterpenes.Angewandte Chemie (International ed. in English) · 2025Article
- From deficiency to toxicity: Magnesium increases cannabinoid and terpene production in cannabis plants.Journal of cannabis research · 2025Article
- Mechanistic Characterisation of a Diterpene Synthase for Chryseojoostenes A-E from Chryseobacterium Joostei.Angewandte Chemie (International ed. in English) · 2025Article
- Exploration and mutagenesis of the germacrene A synthase fromSynthetic and systems biotechnology · 2025Article
- In Silico Genome-Wide Mining and Analysis of Terpene Synthase Gene Family in Hevea Brasiliensis.Biochemical genetics · 2023Article
- Plant terpene specialized metabolism: complex networks or simple linear pathways?The Plant journal : for cell and molecular biology · 2023Review
- Functional characterisation of twelve terpene synthases from actinobacteria.Beilstein journal of organic chemistry · 2023Article
- Transcriptomic Analysis of Resistant and Susceptible Responses in a New Model Root-Knot Nematode Infection System UsingFrontiers in plant science · 2021Article
- Germacrene A-A Central Intermediate in Sesquiterpene Biosynthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Review
- Detoxification of the solanaceous phytoalexins rishitin, lubimin, oxylubimin and solavetivone via a cytochrome P450 oxygenase.Plant signaling & behavior · 2020Article
- An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection.Journal of experimental botany · 2019Article
- Mouse lipogenic proteins promote the co-accumulation of triacylglycerols and sesquiterpenes in plant cells.Planta · 2019Article
- Article
- Terpene Biosynthesis in Red Algae Is Catalyzed by Microbial Type But Not Typical Plant Terpene Synthases.Plant physiology · 2019Article
- P450s controlling metabolic bifurcations in plant terpene specialized metabolism.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2018Article
- An (E,E)-α-farnesene synthase gene of soybean has a role in defence against nematodes and is involved in synthesizing insect-induced volatiles.Plant biotechnology journal · 2017Article
- The Full-Size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 Function in Pre- and Postinvasion Defense against Phytophthora infestans in Nicotiana benthamiana.The Plant cell · 2016Article
- Identification of a Dolabellane Type Diterpene Synthase and other Root-Expressed Diterpene Synthases inFrontiers in plant science · 2016Article
- A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X.Scientific reports · 2015Article
- Plant terpenes: defense responses, phylogenetic analysis, regulation and clinical applications.3 Biotech · 2015Article
9 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initial step in the conversion of the isoprenoid intermediate farnesyl diphosphate to the sesquiterpenoid phytoalexin capsidiol in elicitor-treated tobacco tissues is catalyzed by an inducible sesquiterpene cyclase [5-epi-aristolochene synthase (EAS)]. Two independent cDNA clones (cEAS1 and cEAS2) encoding EAS were isolated from an elicitor-induced tobacco cDNA library by differential hybridization and subsequently were characterized by hybrid selection--in vitro translation. Insertion of cEAS1, a partial cDNA clone encoding 175 C-terminal amino acids, into an Escherichia coli expression vector resulted in accumulation of a fusion protein immunodetectable with EAS-specific polyclonal antibodies. The cDNA clones were used to isolate two full-length EAS genes that mapped 5 kilobases (kb) apart on one 15-kb genomic clone. The nucleotide sequences of the structural gene components were identical from 388 base pairs (bp) upstream of the transcription initiation site to 40 bp downstream of the translation termination codon, suggesting a relatively recent duplication event. The genes consist of 1479-bp open reading frames, each containing five introns and specifying 56,828-Da proteins. The N-terminal amino acid sequence deduced from the genomic clones was identical to the first 16 amino acids of the EAS protein identifiable by Edman degradation. RNA blot hybridization with cEAS1 demonstrated a mRNA induction time course consistent with the induction of the EAS mRNA translational activity with maximum levels 4-6 h after elicitation. EAS mRNA was not detected in control cells. DNA blot-hybridization analysis of genomic DNA revealed a copy number of approximately 12-15 for EAS-like genes in the tetraploid tobacco genome. The conservation of a putative allelic prenyl diphosphate binding motif is also discussed.
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