ArticleProceedings of the National Academy of Sciences of the United States of America1992
Preferential repair of cyclobutane pyrimidine dimers in the transcribed strand of a gene in yeast chromosomes and plasmids is dependent on transcription.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 176 citations in OpenAlex.
- A quantitative modelling approach for DNA repair on a population scale.PLoS computational biology · 2022Article
- RNA polymerase pausing, stalling and bypass during transcription of damaged DNA: from molecular basis to functional consequences.Nucleic acids research · 2022Review
- New insights into abasic site repair and tolerance.DNA repair · 2020Review
- Single-molecule live-cell imaging visualizes parallel pathways of prokaryotic nucleotide excision repair.Nature communications · 2020Article
- From Mfd to TRCF and Back Again-A Perspective on Bacterial Transcription-coupled Nucleotide Excision Repair.Photochemistry and photobiology · 2017Review
- RNA polymerase II depletion promotes transcription of alternative mRNA species.BMC molecular biology · 2016Article
- Sen1, the yeast homolog of human senataxin, plays a more direct role than Rad26 in transcription coupled DNA repair.Nucleic acids research · 2016Article
- Structural insights into the recognition of cisplatin and AAF-dG lesion by Rad14 (XPA).Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Regulation of active genome integrity and expression by Rad26p.Nucleus (Austin, Tex.) · 2014Review
- Mammalian transcription-coupled excision repair.Cold Spring Harbor perspectives in biology · 2013Review
- Review
- Rad26, the transcription-coupled repair factor in yeast, is required for removal of stalled RNA polymerase-II following UV irradiation.PloS one · 2013Article
- Preferential repair of DNA double-strand break at the active gene in vivo.The Journal of biological chemistry · 2012Article
- Rad26p, a transcription-coupled repair factor, is recruited to the site of DNA lesion in an elongating RNA polymerase II-dependent manner in vivo.Nucleic acids research · 2010Article
- A cassette of N-terminal amino acids of histone H2B are required for efficient cell survival, DNA repair and Swi/Snf binding in UV irradiated yeast.Nucleic acids research · 2010Article
- Rapamycin inhibits yeast nucleotide excision repair independently of tor kinases.Toxicological sciences : an official journal of the Society of Toxicology · 2010Article
- Histone H3 Lys79 methylation is required for efficient nucleotide excision repair in a silenced locus of Saccharomyces cerevisiae.Nucleic acids research · 2009Article
- A single amino acid change in histone H4 enhances UV survival and DNA repair in yeast.Nucleic acids research · 2008Article
- ELA1 and CUL3 are required along with ELC1 for RNA polymerase II polyubiquitylation and degradation in DNA-damaged yeast cells.Molecular and cellular biology · 2007Article
- Caloric restriction and genomic stability.Nucleic acids research · 2007Review
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
While preferential repair of the transcribed strands within active genes has been demonstrated in organisms as diverse as humans and Escherichia coli, it has not previously been shown to occur in chromosomal genes in the yeast Saccharomyces cerevisiae. We found that repair of cyclobutane pyrimidine dimers in the transcribed strand of the expressed RPB2 gene in the chromosome of a repair-proficient strain is much more rapid than that in the nontranscribed strand. Furthermore, a copy of the RPB2 gene borne on a centromeric ARS1 plasmid showed the same strand bias in repair. To investigate the relation of this strand bias to transcription, we studied repair in a yeast strain with the temperature-sensitive mutation, rpb1-1, in the largest subunit of RNA polymerase II. When exponentially growing rpb1-1 cells are shifted to the nonpermissive temperature, they rapidly cease mRNA synthesis. At the permissive temperature, both rpb1-1 and the wild-type, parental cells exhibited rapid, proficient repair in the transcribed strand of chromosomal and plasmid-borne copies of the RPB2 gene. At the nonpermissive temperature, the rate of repair in the transcribed strand in rpb1-1 cells was reduced to that in the nontranscribed strand. These findings establish the dependence of strand bias in repair on transcription by RNA polymerase II in the chromosomes and in plasmids, and they validate the use of plasmids for analysis of the relation of repair to transcription in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.