ArticleProceedings of the National Academy of Sciences of the United States of America1992
Construction of chimeric vaccinia viruses by molecular cloning and packaging.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Rapid Generation of Recombinant Poxviruses Using CRISPR/Cas9 Gene Editing.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Development of a multi-antigenic SARS-CoV-2 vaccine candidate using a synthetic poxvirus platform.Nature communications · 2020Article
- Development of a Multi-Antigenic SARS-CoV-2 Vaccine Using a Synthetic Poxvirus Platform.Research square · 2020Article
- Development of a Synthetic Poxvirus-Based SARS-CoV-2 Vaccine.bioRxiv : the preprint server for biology · 2020Article
- Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Synthetic viruses-Anything new?PLoS pathogens · 2018Article
- Construction of an infectious horsepox virus vaccine from chemically synthesized DNA fragments.PloS one · 2018Article
- Reprint of: Coronavirus reverse genetic systems: infectious clones and replicons.Virus research · 2014Article
- Coronavirus reverse genetic systems: infectious clones and replicons.Virus research · 2014Review
- Genome scale patterns of recombination between coinfecting vaccinia viruses.Journal of virology · 2014Article
- Article
- Reverse genetics of coronaviruses using vaccinia virus vectors.Current topics in microbiology and immunology · 2005Review
- High-frequency genetic recombination and reactivation of orthopoxviruses from DNA fragments transfected into leporipoxvirus-infected cells.Journal of virology · 2003Article
- Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Recombinant vaccinia viruses. Design, generation, and isolation.Molecular biotechnology · 1999Article
- Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Foreign DNA was inserted into unique restriction endonuclease cleavage sites (Sma I or Not I) of the 200,000-base-pair vaccinia virus genome by direct molecular cloning. The modified vaccinia virus DNA was packaged in fowlpox virus-infected avian cells, and chimeric vaccinia virus was isolated from mammalian cells not supporting the growth of the fowlpox helper virus. In contrast to the classical "in vivo" recombination technique, chimeric viruses with inserts in both possible orientations and families of chimeras with multiple inserts were obtained. The different genomic configurations of chimeric viruses provide a broader basis for screening of optimal viruses. In addition to packaging in avian cells, a second packaging procedure for vaccinia DNA, based on the abortive infection of mammalian cells with the fowlpox helper virus, was developed. This procedure permits simultaneous packaging and host-range selection for the packaged virus. The cloning/packaging procedure allows the direct insertion of foreign DNA without the need for plasmids having flanking regions homologous to viral nonessential regions and is independent of inefficient in vivo recombination events. By direct cloning and packaging, about 5-10% of the total vaccinia virus yield consisted of chimeras. The procedure is, therefore, a useful tool in molecular virology.
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