ArticleJournal of virology1992
Human immunodeficiency virus type 1 Rev activation can be achieved without Rev-responsive element RNA if Rev is directed to the target as a Rev/MS2 fusion protein which tethers the MS2 operator RNA.
Article in Journal of virology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Contributions of Individual Domains to Function of the HIV-1 Rev Response Element.Journal of virology · 2017Article
- Formation of trans-activation competent HIV-1 Rev:RRE complexes requires the recruitment of multiple protein activation domains.PloS one · 2012Article
- The HIV-1 Rev response element: an RNA scaffold that directs the cooperative assembly of a homo-oligomeric ribonucleoprotein complex.RNA biology · 2012Article
- Rev proteins of human and simian immunodeficiency virus enhance RNA encapsidation.PLoS pathogens · 2007Article
- Reduced mobilization of Rev-responsive element-deficient lentiviral vectors.Journal of virology · 2005Article
- Inhibition of human immunodeficiency virus type 1 Rev function by a dominant-negative mutant of Sam68 through sequestration of unspliced RNA at perinuclear bundles.Journal of virology · 2001Article
- Article
- Polyvalent Rev decoys act as artificial Rev-responsive elements.Journal of virology · 1999Article
- The human immunodeficiency virus type 1 Rev protein and the Rev-responsive element counteract the effect of an inhibitory 5' splice site in a 3' untranslated region.Molecular and cellular biology · 1995Article
- Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.The EMBO journal · 1994Article
- Cellular protein modulates effects of human immunodeficiency virus type 1 Rev.Journal of virology · 1994Article
- Two functional domains of the influenza virus NS1 protein are required for regulation of nuclear export of mRNA.Journal of virology · 1994Article
- Human immunodeficiency virus type 1 Rev-responsive element RNA binds to host cell-specific proteins.Journal of virology · 1994Article
- Functional analysis of interactions between Tat and the trans-activation response element of human immunodeficiency virus type 1 in cells.Journal of virology · 1993Article
- The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assembly.Journal of virology · 1993Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The posttranscriptional trans activation of unspliced or partially spliced human immunodeficiency virus RNAs by the Rev regulatory protein is crucial for virus replication and is dependent on sequence-specific RNA binding by Rev. The cognate RNA target of Rev is contained within a highly structured, 244-nucleotide Rev-responsive element (RRE) RNA in the viral env gene. Here, we show that specific interaction with the RRE is not an absolute requirement for Rev function. When the RRE is replaced by a heterologous MS2 phage operator sequence, Rev will facilitate the cytoplasmic expression of human immunodeficiency virus mRNAs containing this sequence if directed to the MS2 operator via the RNA binding motif of the MS2 phage coat protein (MS-C) as a Rev/MS-C fusion protein. Rev/MS-C efficiently activated both RRE and MS2 targets. A mutation in the MS2 operator that abolished the coat protein binding in vitro rendered the mutant RNA nonresponsive to the fusion protein in vivo. Notwithstanding that Rev can be tethered to the viral RNAs via another RNA binding motif, the structural integrity of the N terminus of Rev was still required for optimal trans activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.