ArticleThe Journal of clinical investigation1992
Alterations in the protein composition of maturing phagosomes.
Article in The Journal of clinical investigation, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed.
- Quantitative Immunofluorescence to Study Phagosome Maturation and Resolution.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Endoplasmic reticulum-Phagosome contact sites from the cradle to the grave.Frontiers in cell and developmental biology · 2022Review
- Review
- Idiosyncratic Biogenesis of Intracellular Pathogens-Containing Vacuoles.Frontiers in cellular and infection microbiology · 2021Review
- The effects of neutrophil-generated hypochlorous acid and other hypohalous acids on host and pathogens.Cellular and molecular life sciences : CMLS · 2021Review
- Alantolactone Enhances the Phagocytic Properties of Human Macrophages and Modulates Their Proinflammatory Functions.Frontiers in pharmacology · 2020Article
- pH of endophagosomes controls association of their membranes with Vps34 and PtdIns(3)P levels.The Journal of cell biology · 2018Article
- The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation.Nature communications · 2017Article
- Arginine Metabolism in Bacterial Pathogenesis and Cancer Therapy.International journal of molecular sciences · 2016Review
- Antimicrobial Mechanisms of Macrophages and the Immune Evasion Strategies of Staphylococcus aureus.Pathogens (Basel, Switzerland) · 2015Review
- Macrophages phagocytose nonopsonized silica particles using a unique microtubule-dependent pathway.Molecular biology of the cell · 2015Article
- Cysteine-mediated redox signaling: chemistry, biology, and tools for discovery.Chemical reviews · 2013Review
- Proteomic characterization of phagosomal membrane microdomains during phagolysosome biogenesis and evolution.Molecular & cellular proteomics : MCP · 2012Article
- Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.Molecular & cellular proteomics : MCP · 2012Article
- Pathogen recognition and activation of the innate immune response in zebrafish.Advances in hematology · 2012Article
- Tuberculosis: new aspects of an old disease.International journal of cell biology · 2011Article
- IL-16 promotes T. whipplei replication by inhibiting phagosome conversion and modulating macrophage activation.PloS one · 2010Article
- The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages.PloS one · 2010Article
- Macrophage NADPH oxidase flavocytochrome B localizes to the plasma membrane and Rab11-positive recycling endosomes.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Phthiocerol dimycocerosates of M. tuberculosis participate in macrophage invasion by inducing changes in the organization of plasma membrane lipids.PLoS pathogens · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
We investigated the protein composition of J774-E clone macrophage phagosomes isolated at different stages of phagolysosome biogenesis. Phagosomes formed by internalizing antibody-coated Staphylococcus aureus for 3 min followed by chase for 0, 4, 9, or 15 min were isolated by density gradient centrifugation. Enrichment and purity of the phagosome preparations were quantitated by radiolabeled ligand recovery, enzyme markers, and electron microscopy. One-dimensional SDS-PAGE analyses of the isolated phagosomes revealed virtually identical protein compositions. However, Western blot analyses with antibodies directed against selected proteins of known itineraries along the endocytic pathway demonstrated distinct differences in phagosome protein compositions. Accumulating within the maturing phagosome were the 31-kD subunit of the vacuolar proton pump, cathepsin D,beta-glucuronidase, the cation dependent mannose 6-phosphate receptor, and LAMP-1. Decreasing within the maturing phagosome were the FcII receptor, the mannose receptor, and alpha-adaptin. These results indicate that although the macrophage phagosome's total protein composition changes little during phagolysosome formation, the maturing phagosome both receives and eliminates, possibly by protein recycling, specific membrane and sequestered proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.