ArticleHistochemistry1992
Which fluorophore is brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, Texas red, and cyanine 3.18.
Article in Histochemistry, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 98 citations in OpenAlex.
- Organic Fluorescent Sensors for Environmental Analysis: A Critical Review and Insights into Inorganic Alternatives.Nanomaterials (Basel, Switzerland) · 2025Review
- Tear-mediated delivery of nanoparticles through transcytosis of the lacrimal gland.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Towards Fluorescence In Vivo Hybridization (FIVH) Detection of H. pylori in Gastric Mucosa Using Advanced LNA Probes.PloS one · 2015Article
- The utility of the DNA microarray scanner to simplify the immunofluorescence evaluation of autoimmune bullous diseases.The American Journal of dermatopathology · 2009Article
- Cy3B: improving the performance of cyanine dyes.Journal of fluorescence · 2004Article
- Detection of Gallibacterium spp. in chickens by fluorescent 16S rRNA in situ hybridization.Journal of clinical microbiology · 2003Article
- Murine salmonellosis studied by confocal microscopy: Salmonella typhimurium resides intracellularly inside macrophages and exerts a cytotoxic effect on phagocytes in vivo.The Journal of experimental medicine · 1997Article
- Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification.Applied and environmental microbiology · 1997Article
- Detection of naturally expressed receptors for gastrin-releasing peptide and tachykinins using cyanine 3-labelled neuropeptides.The Histochemical journal · 1996Article
- Evaluation of five green fluorescence-emitting streptavidin-conjugated fluorochromes for use in immunofluorescence microscopy.Histochemistry and cell biology · 1996Article
- High resolution multicolor fluorescence in situ hybridization using cyanine and fluorescein dyes: rapid chromosome identification by directly fluorescently labeled alphoid DNA probes.Human genetics · 1996Article
- Article
- Sensitivity enhancement of fluorescence in situ hybridization on plant chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
There are several red-emitting fluorophores available for immunofluorescence studies, including tetramethylrhodamine, lissamine rhodamine, Texas Red, and cyanine 3.18; however, it is unclear which of these is best. The present study compared the brightness of these fluorophores to that of fluorescein. Staining was attempted using a primary antibody raised against serotonin and a secondary antibody that was conjugated with either fluorescein or one of the red fluorophores. The intensity of staining was determined densitometrically. It was found that a conjugate of cyanine 3.18 provided significantly brighter staining that conjugates of any of the other fluorophores, including fluorescein. It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.
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