ArticleImmunology1992
Heterogeneity of human blood monocyte: two subpopulations with different sizes, phenotypes and functions.
Article in Immunology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 86 citations in OpenAlex.
- Review
- Molecular Weight-Driven Tunable Hyaluronic Acid-Based Hydrogels Modulate Immune Polarization in Three-Dimensional Microenvironments.ACS biomaterials science & engineering · 2026Article
- Recent Advances in 3D Bioprinting of Porous Scaffolds for Tissue Engineering: A Narrative and Critical Review.Journal of functional biomaterials · 2025Review
- The Role of Monocyte Distribution Width in the Early Prediction of Sepsis in Patients Undergoing Cardiovascular Surgery: A Cross-Sectional Study.Medicina (Kaunas, Lithuania) · 2024Article
- High-frequency and functional mitochondrial DNA mutations at the single-cell level.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- RUV-III-NB: normalization of single cell RNA-seq data.Nucleic acids research · 2022Article
- Fever, Tachypnea, and Monocyte Distribution Width Predicts Length of Stay for Patients with COVID-19: A Pioneer Study.Journal of personalized medicine · 2022Article
- Physical phenotype of blood cells is altered in COVID-19.Biophysical journal · 2021Article
- Leukocyte cell population data from the blood cell analyzer as a predictive marker for severity of acute pancreatitis.Journal of clinical laboratory analysis · 2021Article
- Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry.Nature communications · 2021Article
- Mechanical adaptation of monocytes in model lung capillary networks.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Comparison of Monocyte Distribution Width (MDW) and Procalcitonin for early recognition of sepsis.PloS one · 2020Observational
- White blood cell population dynamics for risk stratification of acute coronary syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Improved Early Detection of Sepsis in the ED With a Novel Monocyte Distribution Width Biomarker.Chest · 2017Article
- Increased Protease-Activated Receptor-2 (PAR-2) Expression on CD14++CD16+ Peripheral Blood Monocytes of Patients with Severe Asthma.PloS one · 2015Article
- Antibody-conjugated paramagnetic nanobeads: kinetics of bead-cell binding.International journal of molecular sciences · 2014Article
- Up-regulation of Toll-like receptors 2 and 4 on peripheral monocytes after major abdominal surgical operation.Digestive diseases and sciences · 2013Article
- A quantitative evaluation of cell migration by the phagokinetic track motility assay.Journal of visualized experiments : JoVE · 2012Article
- Spontaneous and induced osteoclastogenic behaviour of human peripheral blood mononuclear cells and their CD14(+) and CD14(-) cell fractions.Cell proliferation · 2011Article
- Preoperative Toll-like receptor expression in monocytes is associated with host immunological responses following gastrointestinal surgery.World journal of surgery · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human blood mononuclear cells from normal adults were collected after density-cut centrifugation and monocytes were then isolated by removal of lymphocytes using the techniques of E-rosetting and cell adhesion. The purified monocytes were further analysed by velocity sedimentation, and two distinct subpopulations with different cell sizes were obtained. The larger monocytes were 17.0 +/- 1.8 microns in diameter with a mean sedimentation rate (SR) of 7.0 +/- 0.6 mm/hr, while the smaller monocytes were 9.5 +/- 0.8 microns in size and 4.1 +/- 0.2 mm/hr in SR. The population ratio of larger:smaller cells was approximately 2:1 (66 +/- 2.8%:34 +/- 1.6%). Both cell populations exhibited a high positive rate (> 98%) in both the non-specific esterase and the peroxidase stain. However, the larger cells had much higher phagocytic activity than the smaller ones. Furthermore, the expression of monocyte-associated antigens was also different between these two subpopulations. Thus, while most of the larger monocytes (98%) could be recognized by monoclonal antibodies MY7 and OKM1, only some (35 and 61%, respectively) of the smaller monocytes could react with those antibodies. In addition, the larger monocytes secreted a significant amount of monokines including interleukin-1 beta (IL-1 beta), tumour necrosis factor-alpha (TNF-alpha) and prostaglandin E2 (PGE2) and their production increased in proportion to the level of stimulation by bacterial lipopolysaccharide (LPS), whereas the production of monokines by the smaller monocytes remained at low levels and did not respond to LPS stimulation. These results reveal the existence of phenotypic and functional heterogeneity in human blood monocytes.
Indexed as
Identifiers
1427982PMC1421624W107683908What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.