ArticleEuropean biophysics journal : EBJ1992
Expression of a genomic clone encoding a brain potassium channel in mammalian cells using lipofection.
Article in European biophysics journal : EBJ, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 12 citations in OpenAlex.
- Predominant functional expression of Kv1.3 by activated microglia of the hippocampus after Status epilepticus.PloS one · 2009Article
- Molecular template for a voltage sensor in a novel K+ channel. I. Identification and functional characterization of KvLm, a voltage-gated K+ channel from Listeria monocytogenes.The Journal of general physiology · 2006Article
- Mutation of conserved negatively charged residues in the S2 and S3 transmembrane segments of a mammalian K+ channel selectively modulates channel gating.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Expression of a genomic clone encoding a brain potassium channel in mammalian cells using lipofection.European biophysics journal : EBJ · 1992Article
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4 authors at 1 institution in 1 country.
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Abstract
A genomic clone encoding a mouse brain K+ channel (MBK1) was isolated, characterized and expressed in COS cells using the lipofection technique. Transfected COS cells expressed voltage-dependent K+ currents that activated within 20 ms at 0 mV and showed less than 10% inactivation during 250 ms depolarizing pulses at 60 mV. Expressed K+ currents were reversibly blocked by 4-aminopyridine and tetraethylammonium, and were moderately sensitive to dendrotoxin, but insensitive to charybdotoxin. Thus MBK1, expressed transiently in a mammalian cell line, exhibits features characteristic of non-inactivating K+ channels with a conspicuous insensitivity to charybdotoxin. Lipofection is, therefore, a valuable strategy for expression of channel proteins in mammalian cells.
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